The inheritance of a male‐sterile condition in red clover ( Trifolium pratense L.) was determined. Appropriate crosses were made onto a male‐sterile clone, 740, and onto clone 66 which possessed a previously identified malesterile gene (ms 1 ). Segregations for male‐fertility and male‐sterility were observed in the following generations: F 1 , generation, F 1 and F 2 testcrossed to respective parent clones and F 1 's, F 2 generation from sib‐mated F 1 's and sib‐mated F 2 's. All ratios observed indicated a second single recessive gene was responsible for male‐sterility in clone 740. This locus was apparently independent from in ms 1 , of clone 66 and also from the incompatibility factors (S‐alleles). Accordingly, this locus was designated ms 2 to distinguish it from ms 1 . The two loci (ms 1 and ms 2 ) interacted in the sib‐mated F 2 generation to give a duplicate recessive epistatic type of gene action as indicated by a 9:7 ratio.
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