<title>Abstract</title> This protocol outlines reagents and procedures to generate human knock-in organoids using our established strategy named CRISPR-HOT. The protocol describes the plasmids to be used and strategies to generate knock-in organoids from different organs.
Benedetta Artegiani, Delilah Hendriks, Joep Beumer, Rutger N.U. Kok, Xuan Zheng, Indi P. Joore, Susana M. Chuva de Sousa Lopes, Jeroen S. van Zon, Sander J. Tans, Hans Clevers
Victor Tirolle, Adrien Krug, Emma Bokobza, Mattijs Bulcaen, M. Ensinck, Maarten H. Geurts, Delilah Hendriks, F. Vermeulen, Frédéric Larbret, Alejandra Gutiérrez-Guerrero, Louise Medaer, Rik Gijsbers, Philippe Mangeot, Hans Clevers, Marianne S. Carlon, Frédéric Bost, Els Verhoeyen
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