Gel Electrophoresis of DNA --- New Measurements and the Repton Model at High Fields

Faculty of Biotechnology, Jagellonian UniversityGronostajowa 7, 30-387 Cracow, Poland(Received December 7, 2004)The paper is dedicated to Prof. Andrzej Fulinski for his 70th birthdayNew experimental data are presented on the gel electrophoresisofDNA.Experiment was made for molecules of length 173kbp, in 1 percent agarosegel, in TAE 1× buffer and the field intensity between 5 and 9V/cm. Theresults are compared with our computer simulations, performed within therepton model of Duke and Rubinstein. The ranges of field and moleculelength are determined, where the geometration effect appears. We investi-gate also the field dependence of the velocity and the diffusion coefficientat the border of the geometration regime.PACS numbers: 87.15.Tt, 05.10.Ln, 83.10.Kn