Flash-Based Live-Cell Fluorescent Imaging of Synthetic Peptides Expressed in Arabidopsis and Tobacco

José M. Estevez; Chris Somerville

doi:10.2144/000112264

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Flash-Based Live-Cell Fluorescent Imaging of Synthetic Peptides Expressed in Arabidopsis and Tobacco

Article 2006 en

Abstract

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Genes encoding synthetic hydroxyproline-rich peptides with repetitive (Ser-Pro) units linked to an extensin signal sequence and a tetracysteine (TC) sequence were expressed transiently in tobacco, and transiently and stably in Arabidopsis under control of a strong constitutive promoter Expression of these peptides could be visualized in live cells by confocal microscopy following labeling with FlAsH- or ReAsH-EDT2 reagents.

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Flash-Based Live-Cell Fluorescent Imaging of Synthetic Peptides Expressed in <i>Arabidopsis</i> and Tobacco

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