Figure S13 from ERBB4-Mediated Signaling Is a Mediator of Resistance to PI3K and BTK Inhibitors in B-cell Lymphoid Neoplasms

<p>Immunoblotting for total and p-ERBB4, p-AKT and p-ERK in parental (left) and resistant (right) Karpas1718 cells (A-B), and TMD8 cells (C). All blots are representative of two independent experiments. Karpas1718 or TMD8 cells were stimulated with PBS or HBEGF (30ng/mL) for 4 hours, washed-up and treated with DMSO (0.1%), idelalisib (1µM), lapatinib (1µM) or the combination. Levels of phospho proteins were normalized to vinculin and total protein. Data on the barplots represent the mean of two experiments, errors bars represent standard deviation of the mean. * for p<0.05, ** for p<0.01 and ns for not significant from a t-test.</p>