Figure 4 from Cigarette Smoking and E-cigarette Use Induce Shared DNA Methylation Changes Linked to Carcinogenesis

<p>Impact of e-cigarette and smokeless use on cell type–specific epigenetic smoking signatures. <b>A,</b> Mean beta values (corrected) in each of the four sets in saliva samples of never or current smokers or e-cigarette users, corrected for cell type–specific effects. <b>B,</b> AUC of corrected values in each of the four sets comparing smokers or e-cigarette users with controls in the e-cigarette use dataset. <b>C,</b> Mean beta values in each of the four sets in never smokers (control) or smokers, stratified by categorial smoking duration information. <b>D,</b> Mean beta values in each of the four sets in never smokers (control) or e-cigarette users, stratified by categorial e-cigarette duration information. The legend is identical to <b>C</b>. <b>E,</b> Mean beta values (corrected) in each of the four sets in saliva samples of current nonsmokers (prior smoking history not known), smokeless tobacco users, or smokers in the smokeless tobacco use set. <b>F,</b> AUC of corrected values in each of the four sets of CpGs comparing nonsmokers with smokeless tobacco users or smokers in the smokeless tobacco use set. *, <i>P</i> < 0.05; **, <i>P</i> < 0.01; ***, <i>P</i> < 0.001; ****, <i>P</i> < 0.0001 in Wilcoxon test compared with relevant controls (never or nonsmokers, respectively, for <b>A</b>, <b>C</b>, <b>D</b>, and <b>E</b>).</p>