FIGURE 3 from An Early Neoplasia Index (ENI10), Based on Molecular Identity of CD10 Cells and Associated Stemness Biomarkers, is a Predictor of Patient Outcome in Many Cancers

<p>The ENI10 molecular signature is enriched in genes involved in chromosome segregation during mitosis. <b>A,</b> GO analysis on the CD10 signature genes using the “Biological Process” terms. The most downstream terms in the hierarchy with an FDR less than 0.01 are shown. <b>B,</b> Ratio of the percentage of CD10<sup>+</sup> over CD10-negative cells in each phase of the cell cycle determined by flow-cytometry analysis of MCF10A-Fucci-CA cells stained with an anti-CD10 antibody. <b>C,</b> GSEA using RNA-seq experiments comparing MCF10A cells expressing a scramble (shCTRL) or CD10 specific shRNA (shCD10), the gene sets used are the ENI10 genes (top), ENI10 genes belonging to all enriched GO terms shown in A (middle) and ENI10 genes belonging to the “mitotic spindle assembly checkpoint signaling” GO term (bottom). <b>D,</b> ssGSEA quantification of the indicated gene sets in normal healthy breast tissue (H) or in DCIS (D) from GSE21422 series. <b>E,</b> Quantification of E-CFC from CD10<sup>+</sup> MC26 or M1B26 cells infected with lentiviruses carrying a scramble (sh ctl) or sh CD10 vector. <b>F,</b> Quantification of spheres forming cells from CD10<sup>+</sup> MC26 or M1B26 cells infected with lentiviruses carrying a scramble (sh ctl) or sh CD10 vector. <b>G,</b> Quantification of soft-agar clones from M1B26 CD10<sup>+</sup> cells infected with lentiviruses carrying a scramble (sh ctl) or sh CD10 vector.</p>