Femtosecond laser spectroscopy of europium complexes in solutions

The luminescence response of a new coordination complex of europium Eu(III) ion, namely tris[4,4,4-trifluoro-1-(2-thienyl)-1,3-butanediono]Eu(III) 1,4-diaza-bicyclo[2.2.2]octane, applicable in microbiology, biochemistry and molecular biology, is probed upon ultra-high intensity photo-excitation with high-power femtosecond laser pulses. The use of laser light source with ultrashort and high-power pulses should increase by many orders of magnitude the intensity of photoexcitation, and therefore the efficiency of the bioassays and fluorescent microscopy in the research on molecules and structures of biomedical and pharmacological interest, labeled with luminescent complexes. The results obtained show that the luminescence response of the examined compound, even in dilute aqueous solutions is sufficient for fluorometric applications, such as luminescent labels in biomedical diagnostics and bioanalyses, as well as in laser scanning fluorescent microscopy.