Facile preparation of non-self-quenching fluorescent DNA strands with the degree of labeling up to the theoretic limit
Article 2012 en
Authors
YY
Yong Yu
JL
Jianzhao Liu
ZZ
Zujin Zhao
Abstract
1 min read
A new aggregation-induced emission (AIE) compound 1,1-dimethyl-2,5-bis[4-(isothiocyanatemethyl)phenyl]-3,4-diphenylsilole (SITC) was synthesized and used to conjugate with aminoallyl-dUTP. The SITC-dUTP can be incorporated enzymatically into DNA strands with the degree of labeling (DOL) up to the theoretic limit.
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