Establishing a system for the study of urethral biomechanical function

By applying experimental concepts and principles originally established in the study of blood vessel biomechanics, we are exploring various aspects of lower urinary tract function, dysfunction, and treatment effects on tissue properties and performance. We demonstrate here the feasibility of using a modified ex vivo vascular perfusion apparatus for urethral functional studies. Through administration of various pharmacologic agents, and against applied luminal pressures, we demonstrate control over urethral smooth and striated muscle contractile elements. The ability to measure such physiologic responses provides the basis for performing novel functional studies pertaining to the role of each component in overall urethral function and biomechanics. Therefore, the use of this system to evaluate ex vivo physiology and biomechanical properties will advance the understanding of underlying principles of both normal and abnormal urethral function.