Equivalência de técnicas para avaliação da integridade de membrana plasmática em sêmen criopreservado de búfalos (Bubalus bubalis).

This study aimed to determine the association level among laboratorial techniques for assessment of plasma membrane integrity (PMI).Eleven adult buffalo bulls (3.51.0 years and 536.973.0kg) were used as semen donors.Semen was collected using an artificial vagina, totaling one hundred samples (n=100).Semen was cryopreserved in TES-TRIS extender.Further, semen was thawed at 37C during 30 seconds.Each sample was analyzed according the following methods: IP-H342 (propidium iodide 0.5 mg/mL and Hoechst 33342 5 mg/mL); E-N (nigrosin-eosin: 1% eosin B, 5% nigrosin and 3% sodium citrate); and HOST (hypo-osmotic swelling test: 0.735 g sodium citrate, 1.351 g fructose, 100 mL Milli-Q water, 190 mOsm kg -1 ).The IP-H342 was performed in epifluorescent microscopy, E-N was implemented in optical microscopy, while HOST was executed in phase contrast microscopy.Two hundred cells were analyzed in each sample, according different techniques.Data were