Abstract Uric acid is mainly produced from metabolism of purine nucleotide in human body and have several medical importance in human system. It is therefore very imperative to develop a sensitive and accurate method of its detection. This study aimed to develop uric acid biosensor based on polydopamine/polypyrole (PDA/PPY) composite decorated on glassy carbon electrode. The conductive polymer PDA/PPY was electrochemically synthesized and casted on glassy carbon electrode (GCE). The synthesized PDA/PPY was characterized by fourier transform infrared spectroscopy, field emission scanning electron microscopy, electrochemical impedance spectroscopy, energy dispersive x‐ray spectroscopy and x‐ray photoelectron spectroscopy. PDA/PPY/GCE showed good response towards uric acid with low limit of detection (0.1 μM, S/N =3), good linearity (0.5‐40 μM) and high sensitivity (2.1 μAμMcm −2 ). It was also found to have stable response for uric acid unaffected by common interferents. Also, when used for uric acid detection in human serum and urine, it showed a good recovery of 90–110%. The developed PDA/PPY composite is proposed as a promising biosensor for uric acid for human health protection.
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