The present study focuses on the alkaline phosphatase (ALP) mediated formation of apatitic minerals on porous silk fibroin protein (SFP) scaffolds. Porous SFP scaffolds impregnated with different concentrations of ALP are homogeneously mineralized under physiological conditions. The mineral structure is apatite while the structures differ as a function of the ALP concentration. Cellular adhesion, proliferation, and colonization of osteogenic MC3T3 cells improve on the mineralized SFP scaffolds. These findings suggest a simple process to generate mineralized scaffolds that can be used to enhanced bone tissue engineering-related utility.
Sangram Keshari Samal, Mamoni Dash, Tatiana Shelyakova, Heidi Declercq, M. Uhlarz, Manuel Bañobre‐López, Peter Dubruel, Maria Cornelissen, T. Herrmannsdörfer, J. Rivas, G. Padeletti, Stefaan C. De Smedt, Kevin Braeckmans, David Kaplan, V. Dediu
Leping Yan, Joana Silva‐Correia, Mariana B. Oliveira, Carlos Vilela, Hélder Pereira, Rui A. Sousa, João F. Mano, Ana L. Oliveira, Joaquím M. Oliveira, Rui L Reis
Viviana P. Ribeiro, Ana Sofia Ribeiro, Carla J. S. M. Silva, Nelson Durães, Graça Bonifácio, Alexandra P. Marques, Rui A. Sousa, Ana L. Oliveira, Rui L Reis
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