Enhancing Biohybrid CO₂ to Multicarbon Reduction via Adapted Whole-Cell Catalysts

Catalytic CO₂ conversion to renewable fuel is of utmost importance to establish a carbon-neutral society. Bioelectrochemical CO₂ reduction, in which a solid cathode interfaces with CO₂-reducing bacteria, represents a promising approach for renewable and sustainable fuel production. The rational design of biocatalysts in the biohybrid system is imperative to effectively reduce CO₂ into valuable chemicals. Here, we introduce methanol adapted <i>Sporomusa ovata</i> (<i>S. ovata</i>) to enhance the slow metabolic activity of wild-type microorganisms to our semiconductive silicon nanowires (Si NWs) array for efficient CO₂ reduction. The adapted whole-cell catalysts enable an enhancement of CO₂ fixation with a superior faradaic efficiency on the poised Si NWs cathode. The synergy of the high-surface-area cathode and the adapted strain achieves a CO₂-reducing current density of 0.88 ± 0.11 mA/cm², which is 2.4-fold higher than the wild-type strain. This new generation of biohybrids using adapted <i>S. ovata</i> also decreases the charge transfer resistance at the cathodic interface and facilitates the faster charge transfer from the solid electrode to bacteria.