The biodegradation of nonylphenol ethoxylates (NPEOs) surfactant often produces more refractory, toxic and estrogenic intermediates.In this study, simple analytical method for NPEOs determination based on High Performance Liquid Chromatography (HPLC) was developed and complete biodegradation of NPEOs was evaluated by "serum bottle method" that uses direct measure of biogas production in anaerobic condition.The reversed phase C18 column was successfully used for detection of long chain NPEO ave9 (having an average of 9 EO units) and short chain NPEO ave1.5 (having an average of 1.5 EO units) and nonylphenol (NP) at short retention time.However, the coelution of oligomers in reversed phase chromatography is unavoidable, thus the distinction between NPEO oligomers is not possible.In biodegradation study study, the long chain NPEO ave9 was partially mineralized at approximately 37% of the theoretical CH 4 production in anaerobic condition.Nevertheless, the refractory intermediates of NPEO ave1.5 and nonylphenol adversely affects the glucoseutilizing methanogens under anaerobic condition, thereby hampering CH 4 formation.It can be concluded that the anaerobic biodegradation of nonylphenolic compounds proceeded at a very slow rate due to their complex physicochemical properties.
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