Determination of quintozine in gastrodia by hollow fiber-based liquid phase microextraction coupled with gas chromatography

Objective:To develop an analytical method for the determination of quintozine in Gastrodia by hollow fiber-based liquid phase microextraction(HF-LPME)coupled with gas chromatography electron capture detector(GC-ECD).Method:Some main experimental parameters that afect the extraction efficiency were optimized.20 μL of octanol was selected as both the extraction solvent and the impregnation solvent.The extraction was carried out at 30 ℃ temperature with a stirring rate of 500 r·min-1 for 30 min.After extraction,2.0 μL of octanol was then injected into GC for further analysis.DB-1701 capillary(30 m×0.32 mm×0.25 μm)was adopted.The column temperature rise by programming.Injector temperature was 220 ℃ with ECD temperature of detector at 250 ℃.The carrier gas was nitrogen.Result:Under this conditions,the concentration factors for quintozine were 125.The calibration curve was linear over a range of 2-60 ng·L-1(r0.99).The limits of detection(LOD)were 1 ng·L-1(S/N=3)for quintozine.The recoveries were 90.3%-97.2% with relative standard deviations(RSD)between 2.5%-6.1%.Conclusion:The method is sensitive,simple and sutiable for determination of quintozine in Gastrodia.