Determination and Analysis of Promazine Hydrochloride in Chicken by High Performance Liquid

To establish a method for the determination of promazine hydrochloride residue in pork by HPLC. The extraction of promazine hydrochloride from pork tissues by ultrasonic extraction and centrifugal extraction was studied. The peak area of promazine hydrochloride was determined by high performance liquid chromatography. The content of promazine hydrochloride in pork was calculated by standard curve equation. The mobile phase was acetonitrile-ammonium acetate (0.02mol/mL) with a ratio of 60:40, the detection wavelength was set at 258 nm, the flow rate was 1.0mL/min, and the sample size was 10μL. The column temperature was 20℃. Acetonitrile was selected as the extraction liquid, extracted by ultrasonic shock method and centrifugal method, purified by solid phase extraction method, and dried and concentrated by vacuum as the pre-treatment method of pork. The results showed that promazine hydrochloride showed a good linear relationship with the peak area in the concentration range of 1ug/mL~50ug/mL (r2=0.9983). The intra-day and intra-day precision were all less than 3%. The recoveries of three different concentrations were 79.31%~84.38%. The method used in this experiment is simple, easy to operate, high accuracy and good reproducibility, which is suitable for the analysis and detection of the residual amount of promazine hydrochloride in pork.