Contrasting effects of arsenic on mycorrhizal-mediated silicon and phosphorus uptake by rice

Silicon (Si) and arbuscular mycorrhizal fungi (AMF) increase plant resistance to various environmental stresses, including heavy metal (and metalloid) toxicity. Although Si and AMF each independently enhance plant tolerance, the nature of their interactions and their combined impacts on nutrient uptake, especially in the context of toxic elements such as arsenic (As), remains to be elucidated. This study investigated AMF-mediated regulation of plant nutrient uptake under As stress using rice, a model Si-accumulating plant. Experiments were conducted under As-free and As stress conditions, incorporating AMF inoculation and silicic acid application, with a focus on nutrient uptake and transporter expression. Without As, AMF inoculation increased shoot Si content by 44%, while invariance was common under As toxicity stress (10 μM of As(III)). Despite As presence, AMF increased Lsi1 expression with Si application, elevating As content in roots and shoots by 38% and 55%, respectively. Introduction of As stress amplified AMF role in phosphorus (P) uptake from 13% to 38%, correlating with up-regulated P transporter expression. Three-way ANOVA of interactions among As, Si, and AMF on P and As uptake by rice revealed that As amplified AMF potential to increase P uptake while weakening promotive effect on Si uptake. Silicon reduced As absorption, while AMF increased As uptake, but the elevated As were potentially retained within fungal hyphae, limiting transfer to rice plants. Overall, As toxicity stress had contrasting effects on P- and Si-promoting roles of AMF. These findings contribute to our understanding of plant-fungal interactions under heavy metal stress.