Complexation and oxidation of Flutamide with Fe 3+ and 1,10-phenanthroline: Few analytical applications

A simple and sensitive spectrophotometric method has been proposed for the determination of flutamide (FLD). The method is based on its complexation and oxidation of FLD by Fe3+ and 1,10-phenanthroline. Two sets of condition were established. In the first set only FLD reacted with Fe3+ ion, while in the second one FLD was oxidized by Fe3+ in the presence of 1,10-phenanthroline. The complexation and oxidation methods were monitored spectrophotometrically. All variables affecting the development of the color were investigated and the conditions obeying Beer’s law were optimized. Plots of absorbance against concentration in both methods were rectilinear over the ranges 0.5–10 and 0.5–1.5 μg mL−1, with mean recoveries of 99.51 and 99.83 for methods A and B respectively. The limits of detection for procedures 1 and 2 are 0.332 and 0.726 μg mL−1 1, respectively. Both the methods were successfully applied for the determination of the FLD in its dosage form.