Collagen‐Like Peptide as a Matrix for Enzyme Immobilization in Electrochemical Biosensors

Abstract An efficient enzyme immobilization technique is described in which well‐organized collagen peptide monolayers are used as a scaffold upon which horseradish peroxidase is anchored on gold electrode surfaces. The resulting electrode presents reproducible amperometric responses at an applied potential of −0.1 V with a range of linearity for peroxides. These results suggest that triple helical collagen can be used for specific immobilization of HRP with advantages of low price, simplicity and biocompatibility. The results are significant for the control of biomolecular self‐assembly in the intrinsic electric devices.