1,5,9-Epideoxyloganic acid (ELA) was isolated from the methanol fraction of endemic Nepeta aristata crude methanol-chloroform by chromatographic methods (preparative-HPLC, silica, and sephadex column). The structure of ELA was determined with 1H NMR and 13C NMR spectrometry. Furthermore, the mass of the molecule was confirmed by mass spectrometry. ELA's antioxidant and antibacterial activities were examined and also compared to standards. ELA exhibited the best antibacterial activity with inhibition zone against K. pneumoniae (11.50 mm), S. aureus (10.00 mm), and also effective minimum inhibition concentration value against E. faecalis (8 µg/mL). The antioxidant properties of ELA were assessed with posphomolybdenium reducing, reducing power, metal chelating, H2O2, DPPH˙, ABTS˙⁺, and O2˙ˉ scavenging activities. The posphomolybdenium, O2˙ˉ, and H2O2 scavenging activities of ELA were found effective at 136.02±3.99, 3.54±0.26 and 9.67±1.26 µg/mL, respectively. As a result, the effective antioxidant and antimicrobial activity values of the ELA molecule may be a source that can be used in pharmacology in the future.
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