Analysis of the squalestatins by on‐line packed capillary liquid chromatography/dynamic liquid secondary‐ion/mass spectrometry

Abstract A mixture of four squalestatins was separated and characterized by gradient‐packed capillary liquid chroma‐tography combined with dynamic liquid secondary‐ion mass spectrometry (LSIMS). Thioglycerol/glycerol matrix was added post‐column. Negative‐ion LSIMS was found to afford spectra typified by strong molecular ions with structurally informative fragments. The new method showed improvements in sensitivity and specificity for this class of compound when compared to existing thermospray methods.