Aerosol delivery of lentivirus-mediated O-glycosylation mutant osteopontin suppresses lung tumorigenesis

Background Osteopontin (OPN) is a secreted glycophosphoprotein that has been implicated in the regulation of cancer development. The function of OPN is primarily regulated through post-translational modification such as glycosylation. As yet, however, the relationship between OPN glycosylation andlungcancerdevelopmenthasnotbeeninvestigated.Inthis study, we addressed this issue by studying the effect of a triple mutant (TM) of OPN, which is mutated at three Oglycosylation sites, on lung cancer development in K-ras LA1 mice, a murine model for human non-small cell lung cancer. Methods Aerosolized lentivirus-based OPN TM was delivered into the lungs of K-ras LA1 mice using a nose-onlyinhalation chamber 3 times/wk for 4 wks. Subsequently, the