(A) RNA was collected from LoVo, A549, HeLa, and Sk-Mel-2 cells transduced with shRNA against SEMA3B (to suppress gene expression; shSEMA3B) and unrelated sequences (shCTR)

Real-time PCR was performed using Taqman probes for SEMA3B (results shown at the bottom) and IL-8 (in the graph). Expression levels were normalized to controls for each cell line and are represented as fold changes. Data shown are the mean ± SEM. (B) Monocyte migration to CM derived from Sk-Mel-2 melanoma cells transduced with shRNAs. IL-8 neutralizing antibodies (MAB208) or unrelated control antibodies (anti-CD19) were included in the lower chamber. Knock down of SEMA3B expression in Sk-Mel-2 cells abolished IL-8–dependent monocyte-attracting activity in the CM. The number of migrated monocytes represents the mean ± SEM of two independent experiments. **, P &lt; 0.01.<b>Copyright information:</b>Taken from "The tumor suppressor semaphorin 3B triggers a prometastatic program mediated by interleukin 8 and the tumor microenvironment"The Journal of Experimental Medicine 2008;205(5):1155-1171.Published online 12 May 2008PMCID:PMC2373847.