A comparative study on the effects of adipose tissue derived and bone marrow mesenchymal stem cells on neurons/glial cells viability, proliferation and differentiation

[Excerpt] It is known that both Mesenchymal Stem Cells (MSCs)and Adipose derived Stem Cells (ASCs) are able to ameliorate the CNS condition upon injury. However it is still not clear whether they have the similar or opposite effects on the different CNS derived cell populations. In this sense the objective of the present work was to understand if ASCs and MSCs preferentially act on different CNS derived cell populations. Hippocampal neurons and glial cells were exposed to MSCs and ASCs conditioned media (CM) (obtained 24, 48, 72 and 96 after 3 days of culture of HUCPVCs) for 1 week. Cell viability experiments (MTS test) revealed that CM obtained for both cell populations at all time points did not cause any deleterious effects on neurons and glial cells. [...]