474 LARGE ACELLULAR SILK SCAFFOLDS SUPPORT ROBUST BLADDER TISSUE REGENERATION IN A PORCINE MODEL OF BLADDER AUGMENTATION

You have accessJournal of UrologyPediatrics: Neuropathic Bladder/Reconstruction1 Apr 2013474 LARGE ACELLULAR SILK SCAFFOLDS SUPPORT ROBUST BLADDER TISSUE REGENERATION IN A PORCINE MODEL OF BLADDER AUGMENTATION Duong Tu, Yeun Goo Chung, Debra Franck, Eun Seok Gil, Abhishek Seth, Pablo Gomez, Rosalyn Adam, David Kaplan, Carlos Estrada, and Joshua Mauney Duong TuDuong Tu Boston, MA More articles by this author , Yeun Goo ChungYeun Goo Chung Boston, MA More articles by this author , Debra FranckDebra Franck Boston, MA More articles by this author , Eun Seok GilEun Seok Gil Medford, MA More articles by this author , Abhishek SethAbhishek Seth Boston, MA More articles by this author , Pablo GomezPablo Gomez Boston, MA More articles by this author , Rosalyn AdamRosalyn Adam Boston, MA More articles by this author , David KaplanDavid Kaplan Medford, MA More articles by this author , Carlos EstradaCarlos Estrada Boston, MA More articles by this author , and Joshua MauneyJoshua Mauney Boston, MA More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.1866AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Biomaterials derived from Bombyx mori silkworms represent an alternative for bladder tissue engineering given their mechanical robustness, processing plasticity, and biodegradability. Our previous results have shown that acellular silk scaffolds support tissue regeneration in a murine model of bladder augmentation. We hypothesize that acellular silk matrices will mediate tissue regeneration in a large animal model of bladder augmentation. METHODS Scaffolds (6x6cm2) were generated from 6% silk fibroin solutions using a solvent casting/sodium chloride-leaching process. Matrices were anastomosed to the bladder dome of Yorkshire pigs (N=6) and maintained for 3 months. Cystometry was used to determine bladder capacity both pre-operatively and at 3 months. Regenerated tissues were evaluated by histological (H&E and Masson's trichrome) and immunohistochemical (IHC) analyses for smooth muscle contractile proteins (α-actin and SM22α), urothelial (cytokeratins, p63, uroplakins), innervation (Fox3) and vascularization (CD31) markers. RESULTS Following scaffold implantation, 5/6 swine survived to the scheduled 3 month euthanasia while 1 animal was sacrificed within the first week due to a urine leak. Following 1 week of initial urinary diversion via urethral catheter, animals were capable of voluntary voiding. Cystometry at 3 months revealed substantial increases (>2-fold) in bladder capacity in 5/6 augmented pigs in comparison to pre-op values and weight-matched unoperated controls. Histological and IHC evaluations of both the periphery and central regions of the regenerated tissues demonstrated robust smooth muscle formation as well as a multi-layered urothelium, similar to nonsurgical control regions. Innervation and vascularization markers were also apparent in the regenerated tissue. CONCLUSIONS Acellular bi-layer silk scaffolds represent an effective biomaterial for bladder tissue regeneration in a large animal model and challenge the convention that large constructs require cell seeding. These constructs may offer advantages over conventional gastrointestinal segments and previously described cellularized biomaterials for augmentation cystoplasty. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e195 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Duong Tu Boston, MA More articles by this author Yeun Goo Chung Boston, MA More articles by this author Debra Franck Boston, MA More articles by this author Eun Seok Gil Medford, MA More articles by this author Abhishek Seth Boston, MA More articles by this author Pablo Gomez Boston, MA More articles by this author Rosalyn Adam Boston, MA More articles by this author David Kaplan Medford, MA More articles by this author Carlos Estrada Boston, MA More articles by this author Joshua Mauney Boston, MA More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...