Publications

Additional file 16. Table S15

The Burnout Assessment Tool (BAT) has been gaining increased attention as a sound and innovative instrument in its conceptualization of burnout. BAT has been adapted for several countries, revealing promising validity evidence. This paper aims to present the psychometric properties of the Brazilian and Portuguese versions of the BAT in both the 23-item and 12-item versions. BAT’s validity evidence based on the internal structure (dimensionality, reliability, and measurement invariance) and validity evidence based on the relations to other variables are the focus of research. A cross-sectional study was conducted with two non-probabilistic convenience samples from two countries (N = 3103) one from Brazil (nBrazil = 2217) and one from Portugal (nPortugal = 886). BAT’s original structure was confirmed, and it achieved measurement invariance across countries. Using both classic test theory and item response theory as frameworks, the BAT presented good validity evidence based on the internal structure. Furthermore, the BAT showed good convergent evidence (i.e., work engagement, co-worker support, role clarity, work overload, and negative change). In conclusion, the psychometric properties of the BAT make this freely available instrument a promising way to measure and compare burnout levels of Portuguese and Brazilian workers.

Many tissue cDCs are maintained as single subset clones, which disperse upon infection with acute recruitment of pre-cDCs.

Abstract The most successful genetically encoded calcium indicators (GECIs) employ an intensity or intensiometric readout. Despite a large calcium-dependent change in fluorescence intensity, the quantification of calcium concentrations with GECIs is problematic, which is further complicated by the sensitivity of all GECIs to changes in the pH in the biological range. Here, we report on a novel sensing strategy in which a conformational change directly modifies the fluorescence quantum yield and fluorescence lifetime of a circular permutated turquoise fluorescent protein. The fluorescence lifetime is an absolute parameter that enables straightforward quantification, eliminating intensity-related artifacts. A new engineering strategy that optimizes lifetime contrast led to a biosensor that shows a 3-fold change in the calcium-dependent quantum yield and a fluorescence lifetime change of 1.3 ns. Additionally, the response of the calcium sensor is insensitive to pH between 6.2-9. As a result, the turquoise GECI enables robust measurements of intracellular calcium concentrations by fluorescence lifetime imaging. We demonstrate quantitative imaging of calcium concentration with the turquoise GECI in single endothelial cells and human-derived organoids. Abstract Figure

Methodology of the overall project - Qualitative studies: Sample criteria - Qualitative studies: comparison of results - Case studies - Case studies of outplacement/replacement interventions - general discussion

The responses of individual organoid to different treatments (irradiation, 5-Fu and CPT-11): data for Patient-derived organoids predict chemoradiation responses of locally advanced rectal cancer.

Significance The stem cell niche is the in vivo microenvironment in which stem cells both reside and receive stimuli that determine their fate. Intestinal Paneth cells produce an arsenal of molecules involved in numerous biological processes, including critical support of the intestinal stem cell niche via the expression of niche ligands, growth factors, and cytokines. The relevance of the niche function of Paneth cells has been challenged, however. Here we show that specific, acute ablation of Paneth cells in mice does not affect the stem cell population, because alternative niche cells derive enteroendocrine and tuft cells. These cells serve as an alternative source of Notch signals, which are essential for intestinal stem cell maintenance.