Publications

The Satisfaction and Frustration of Basic Psychological Needs in Engaging Leadership

The purpose of the current study was to test engaging leadership, which is a recently developed leadership concept based on basic psychological needs theory. Drawing on self‐determination theory, the current study hypothesized that basic psychological needs mediate the relationship between engaging leadership and both positive and negative outcomes. An association between need satisfaction and positive results and an association between need frustration and adverse outcomes were expected. The survey data, collected from three comparable groups from two multinational, technical engineering organizations ( N = 304), were analyzed using partial least squares structural equation modeling. Autonomy satisfaction was found to play a pivotal role in the leadership model and was associated with increased positive outcomes, such as work engagement and autonomous motivation, and decreased unfavorable motivational consequences, such as controlled motivation and amotivation. The highlighted role of autonomy satisfaction informs leaders to focus on the positive aspects of leadership and need fulfillment rather than aiming to diminish frustration. Additionally, the current study adds to the growing body of knowledge on effective leadership approaches in a fast‐changing complex business world and on the benefits of autonomy‐supportive work environments.

Casting a Wider Net: On the Utilitarian Nature of Burnout Assessment in the Workplace

Some consider the burnout label to be controversial, even calling for the abandonment of the term in its entirety. In this communication, we argue for the pragmatic utility of the burnout paradigm from a utilitarian perspective, which advocates the greatest good for the most significant number of employees in organisations. We first distinguish between mild work-related burnout complaints and more severe burnout that can be identified in some contexts. We address the classification of burnout as an ‘occupational phenomenon’ by the World Health Organization and its ambiguous status in the ICD-11, highlighting the challenge of universally diagnosing burnout as a condition. We argue that a purely clinical approach might be too reactive as it normally only identifies employees with a diagnosable condition. We posit that early detection of burnout through valid assessment can identify struggling employees who do not yet have a diagnosable condition. This proactive approach can help prevent escalation into mental health crises and is more sensible for organisations in terms of effectiveness and employee retention.

assay information of the unclassified samples for HeliScopeCAGE

a tab delimited flat file (SDRF file) describing the experimental details for unclassified samples for the standard protocol of the HeliScopeCAGE protocol

Supplemental Figure Legends from Organoid Profiling Identifies Common Responders to Chemotherapy in Pancreatic Cancer

<p>Supplemental Figure Legends</p>

Profiling proliferative cells and their progeny in damaged murine hearts

The significance of cardiac stem cell (CSC) populations for cardiac regeneration remains disputed. Here, we apply the most direct definition of stem cell function (the ability to replace lost tissue through cell division) to interrogate the existence of CSCs. By single-cell mRNA sequencing and genetic lineage tracing using two Ki67 knockin mouse models, we map all proliferating cells and their progeny in homoeostatic and regenerating murine hearts. Cycling cardiomyocytes were only robustly observed in the early postnatal growth phase, while cycling cells in homoeostatic and damaged adult myocardium represented various noncardiomyocyte cell types. Proliferative postdamage fibroblasts expressing follistatin-like protein 1 (FSTL1) closely resemble neonatal cardiac fibroblasts and form the fibrotic scar. Genetic deletion of Fstl1 in cardiac fibroblasts results in postdamage cardiac rupture. We find no evidence for the existence of a quiescent CSC population, for transdifferentiation of other cell types toward cardiomyocytes, or for proliferation of significant numbers of cardiomyocytes in response to cardiac injury.

Lack of reciprocity among Dutch teachers: Validation of reciprocity indices and their relation to stress and well-being

This research presents the results of two related studies on the convergent and construct validity of three measures of reciprocity in exchange relationships at work. In Study 1, 71 Dutch teachers were interviewed about their specific investments and outcomes in the exchange relationships with their students, colleagues and school. ANOVA revealed that they reported significantly more investments than outcomes, and that the number of reported investments and outcomes mentioned varied as a function of the type of exchange relationship. Building on these results, multi-item scales were created to assess reciprocity at a detailed level for each of the three exchange relationships. Study 2 validated these specific reciprocity measures by relating them to two global assessments of reciprocity (convergent validity) as well as to measures of job stress and well-being (construct validity). LISREL-analysis of data obtained from a further sample of 224 teachers revealed that for each type of exchange relationship there were significant, consistent and meaningful relationships among the three reciprocity measures. Further, hierarchical regression analysis showed that the reciprocity measures were differentially related to job stressors and measures of well-being. Implications are discussed. Keywords: ReciprocityEquity TheoryMeasurementTeachersWork Stress

An FBXW7-ZEB2 axis links EMT and tumour microenvironment to promote colorectal cancer stem cells and chemoresistance

Genome-wide CRISPR screens reveal a Wnt–FZD5 signaling circuit as a druggable vulnerability of RNF43-mutant pancreatic tumors

National Heart, Lung, and Blood Institute and Building Respiratory Epithelium and Tissue for Health (BREATH) Consortium Workshop Report: Moving Forward in Lung Regeneration

The National Heart, Lung, and Blood Institute of the National Institutes of Health, together with the Longfonds BREATH consortium, convened a working group to review the field of lung regeneration and suggest avenues for future research. The meeting took place on May 22, 2019, at the American Thoracic Society 2019 conference in Dallas, Texas, United States, and brought together investigators studying lung development, adult stem-cell biology, induced pluripotent stem cells, biomaterials, and respiratory disease. The purpose of the working group was <i>1</i>) to examine the present status of basic science approaches to tackling lung disease and promoting lung regeneration in patients and <i>2</i>) to determine priorities for future research in the field.

Teacher Burnout and Lack of Reciprocity¹

Results are presented of a study on burnout among 249 Dutch elementary and secondary school teachers. The current study considers burnout in terms of the exchange of investments and outcomes at interpersonal (teacher‐student) and organizational (teacher‐school) levels. In addition, demographic (age and gender) and work‐related factors (school type, teaching experience, number of hours employed) are included. Findings show that when teachers invest more than they get back from their school, they report higher levels of emotional exhaustion. As expected, at the interpersonal level, low outcomes from students are related to higher burnout levels; whereas at the organizational level, low investments are related to higher burnout levels. These findings are discussed in terms of the extent to which psychological contact is more intimate or impersonal in both types of relationships.

Fig S4 from &lt;i&gt;Frizzled-7&lt;/i&gt; Is Required for Wnt Signaling in Gastric Tumors with and Without &lt;i&gt;Apc&lt;/i&gt; Mutations

&lt;p&gt;Figure S4. Fzd7 inhibition is rescued by Fzd7 expression in GC cells. Fzd7 deletion in gp130F/F mice reduces adenoma burden and induces cell differentiation and changes to Wnt target gene transcription.&lt;/p&gt;

Correction: The Leukemia-Associated Mllt10/Af10-Dot1l Are Tcf4/β-Catenin Coactivators Essential for Intestinal Homeostasis

[This corrects the article DOI: 10.1371/journal.pbio.1000539.].

Oncogenic signaling is coupled to colorectal cancer cell differentiation state

Colorectal cancer progression is intrinsically linked to stepwise deregulation of the intestinal differentiation trajectory. In this process, sequential mutations of APC, KRAS, TP53, and SMAD4 enable oncogenic signaling and establish the hallmarks of cancer. Here, we use mass cytometry of isogenic human colon organoids and patient-derived cancer organoids to capture oncogenic signaling, cell phenotypes, and differentiation states in a high-dimensional single-cell map. We define a differentiation axis in all tumor progression states from normal to cancer. Our data show that colorectal cancer driver mutations shape the distribution of cells along the differentiation axis. In this regard, subsequent mutations can have stem cell promoting or restricting effects. Individual nodes of the cancer cell signaling network remain coupled to the differentiation state, regardless of the presence of driver mutations. We use single-cell RNA sequencing to link the (phospho-)protein signaling network to transcriptomic states with biological and clinical relevance. Our work highlights how oncogenes gradually shape signaling and transcriptomes during tumor progression.

The Motivational Make-Up of Workaholism and Work Engagement: A Longitudinal Study on Need Satisfaction, Motivation, and Heavy Work Investment

Drawing on Ryan and Deci's Self-Determination Theory, this study examines longitudinally how need satisfaction at work affects four forms of intrinsic and extrinsic work motivation and two types of heavy work investment (workaholism and work engagement). Using two-wave data from 314 Dutch employees, structural equation modeling supported our expectations that high need satisfaction was longitudinally associated with low levels of external and introjected regulation, and high levels of identified regulation and intrinsic motivation. Interestingly, none of these forms of regulation predicted later levels of work engagement and workaholism. Rather, high levels of work engagement predicted later high levels of intrinsic motivation and identified regulation, and high levels of workaholism predicted later low levels of intrinsic motivation and high levels of introjected regulation. Although this study did not support the expected longitudinal effects of motivation on the two types of heavy work investment examined in this study, it (a) underlined the important role of need satisfaction for motivation, (b) challenged previous ideas on the effects of motivation on workaholism and work engagement, and (c) revealed the different motivational correlates of work engagement and workaholism.

Inequity Among Human Service Professionals: Measurement and Relation to Burnout

This research investigated the impact on burnout of inequity experienced by human service professionals. Two studies were conducted, among therapists working with inmates in a forensic psychiatric center (N = 112) and among staff members of an institute for the direct care of mentally disabled (N = 189). Two types of inequity were examined: interpersonal inequity in the relations with recipients and inequity in relation with the organization. The majority of the professionals in both studies felt underbenefited in relation with recipients as well as in relation with their organization. As expected, inequity was curvilinearly related to burnout, especially with the emotional exhaustion dimension. Surprisingly, professionals who felt overbenefited experienced more burnout than colleagues who felt underbenefited. No gender effects were found for the distribution of inequity nor for the relation between inequity and burnout.

Psychometric properties of the Russian version of the utrecht Work engagement scale (UWES-9)

This article aims to analyze the psychometric properties of the Russian version of the Utrecht Work Engagement Scale (UWES-9) by using a sample of 1783 employees of a large Russian organization. We conducted a series of Confirmatory Factor Analysis (CFA) tests of the factorial structure and the configural, metric, scalar, factor variance, factor covariance, and factor means invariances of the model across gender and age groups. The validity of the Russian version of the UWES-9 was investigated by study- ing its relationship with burnout, job satisfaction, life satisfaction, and turnover inten- tion. The CFA showed that both the 1-factor and the 3-factor models of the UWES-9 fit well with the data, but the 3-factor model demonstrates a significantly better fit. however, the differences are minor; therefore, in practical terms, the models are iden- tical. Thus the Russian version of the UWES-9 can be used both as a three-scale mea- surement and as a single scale of work engagement. Results from the multi-group CFA provided evidence of factorial invariance of the 3-factor model across gender and age groups. Internal consistencies of the vigor, dedication, and absorption subscales are acceptable. The results also suggested the convergent, discriminant, and incremental validity of the UWES-9, as shown by its relationship with burnout, job satisfaction, life satisfaction, and turnover intention. The article also discusses the differences in levels of work engagement within different gender and age groups. Overall, the results show that the Russian version of the UWES-9 demonstrates acceptable psychometric properties comparable with the original version and those in other languages. The Russian version of the UWES-9 can therefore be recommended for use in research and practice.

Investigating the relationship between workaholism and workplace aggressive behavior

No abstract is provided for this article.

Human organoid systems reveal <i>in vitro</i> correlates of fitness for SARS-CoV-2 B.1.1.7

Abstract A new phase of the COVID-19 pandemic has started as several SARS-CoV-2 variants are rapidly emerging globally, raising concerns for increased transmissibility. As animal models and traditional in vitro systems may fail to model key aspects of the SARS-CoV-2 replication cycle, representative in vitro systems to assess variants phenotypically are urgently needed. We found that the British variant (clade B.1.1.7), compared to an ancestral SARS-CoV-2 clade B virus, produced higher levels of infectious virus late in infection and had a higher replicative fitness in human airway, alveolar and intestinal organoid models. Our findings unveil human organoids as powerful tools to phenotype viral variants and suggest extended shedding as a correlate of fitness for SARS-CoV-2. One-Sentence Summary British SARS-CoV-2 variant (clade B.1.1.7) infects organoids for extended time and has a higher fitness in vitro .