1,592 publications from this institution
Background: Psychosocial work characteristics are associated with all-cause long-term sickness absence (LTSA).Aims: This study investigated whether psychosocial work characteristics such as higher workload, faster pace of work, less variety in work, lack of performance feedback, and lack of supervisor support are prospectively associated with higher LTSA due to mental disorders.Methods: Cohort study including 4877 workers employed in the distribution and transport sector in The Netherlands. Psychosocial work characteristics were included in a logistic regression model estimating the odds ratios (OR) and 95% confidence intervals (CI) of mental LTSA during 2-year follow-up. The ability of the regression model to discriminate between workers with and without mental LTSA was investigated with the area under the receiver operating characteristic curve (AUC).Results: Tow thousand seven hundred and eighty-two (57%) workers were included in the analysis; 73 (3%) had mental LTSA. Feedback about one’s performance (OR = 0.82; 95% CI 0.70–0.96) was associated with mental LTSA. A prediction model including psychosocial work characteristics poorly discriminated (AUC = 0.65; 95% CI 0.56–0.74) between workers with and without mental LTSA.Conclusions: Feedback about one’s performance is associated with lower rates of mental LTSA, but it is not useful to measure psychosocial work characteristics to identify workers at risk of mental LTSA.
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No abstract is provided for this article.
The human gut, our primary digestive organ, has recently garnered recognition as the largest endocrine organ, characterised by its substantial surface area and the abundance of endocrine cells it harbours.1 Within the gut epithelium, enteroendocrine cells (EECs) represent a specialized cell type dedicated to endocrine functions. At least a half dozen EEC subtypes are recognized that collectively secrete over 20 distinct hormones, which control feeding behaviour, gut motility, insulin levels and other aspects of metabolism (Figure 1). Notably, the L-cells, a specific EEC subtype, produce incretin hormones such as glucagon-like peptide-1 (GLP-1) and peptide YY (PYY), both implicated in the regulation of insulin secretion and glucose homeostasis.2, 3 The clinical significance of EECs is evident in the therapeutic applications of GLP-1 receptor agonists (GLP-1RAs) for type 2 diabetes and obesity. To date, nine GLP-1RAs have received approval from the US Food and Drug Administration (FDA), with numerous others undergoing clinical trials. Similar to other differentiated cell lineages within the intestinal epithelium, EECs originate from regionally-specified Lgr5+ intestinal stem cells (ISCs).4-6 The differentiation of EEC lineages from ISCs is orchestrated by a network of transcription factors (TFs). A prominent player in this regulatory network is NEUROG3, recognized as a master regulator of the endocrine cell development in both the pancreas and the gut.7, 8 By tagging a time-resolved reporter with mouse Neurog3, a real-time and lineage-specific map of mouse EEC differentiation at a single-cell level was constructed.9 This study discovered six novel regulators (Sox4, Rfx6, Tox3, Myt1, Runx1t1 and Zcchc12) involved in EEC differentiation at different stages. Alternatively, overexpression of NEUROG3 has been used as a tool for generating EECs from ISCs, facilitating in-depth profiling of human EECs.10 Nevertheless, the upstream regulators of NEUROG3 and the downstream effectors of NEUROG3 governing EEC subtype differentiation is not fully explored. To achieve a more comprehensive understanding of human EEC differentiation, it is crucial to establish an in vitro model faithfully recapitulating the lineage commitment process. The advert of the organoid technology has revolutionized in vitro culture models for biomedical research. In 2009, our group pioneered the intestinal organoid culture, creating the first three-dimensional self-organizing tissue in vitro derived from adult stem cells. Intestinal organoids faithfully mimic many aspects of the original tissue physiology, including structural complexity and self-driven cell fate decisions. Recently, we further refined an optimal culture system for human intestinal organoids, enabling the spontaneous generation of all major differentiated cell lineages, including EECs, without ectopic overexpression of TFs.11 This culture model provides a more physiologically relevant in vitro model of human adult endocrine differentiation. In our recently published study, we integrated the optimal human gut organoid model with the CRISPR-based genetic screen approach to comprehensively explore the entire repertoire of human TFs involved in EEC differentiation (Figure 2A).12 This organoid-based genetic screening utilised a knockout library comprising 7210 sgRNAs targeting 1800 human TFs, collectively referred to as the TFome. The CRISPR knockout library was first introduced into human small intestinal organoids in the conventional expansion culture by lentiviral transduction. This strategy enabled the identification of 135 essential genes for ISCs, including KLF5 and TCF7L2, whose knockout resulted in impaired cell growth and eventual depletion from the population. Utilising our well-established differentiation protocol for human intestinal organoids, we enriched EECs from differentiated organoids using the endogenous knock-in reporter. After assessing the enrichment of sgRNA construct within EECs, we confirmed the essentiality of the TFs (NEUROG3, SOX4, INSM1) classically known to govern EEC differentiation. We further discovered a few TFs (NFIC, TEF, ZHX2) that modulate EEC differentiation, which were not described previously. Key to our study, we identified a previously unstudied TF, ZNF800, as a robust repressor of EEC differentiation. ZNF800 is a C2H2-zinc finger protein, which is ubiquitously expressed throughout the body at low levels. In our study, we demonstrate that the knockout of ZNF800 in human intestinal organoids (both ileum and colon) causes pronounced increase in EEC numbers (from ∼1% to ∼15%) (Figure 2B). The robust induction of EECs was accompanied by a significant ablation of other secretory cell types (mainly goblet and Paneth cells). This suggests a shift in the cell fate of ISC differentiation upon ZNF800 loss. Notably, the increased EECs were predominantly identified as enterochromaffin cells (ECs), a specific EEC subtype known for producing serotonin (5-HT). This finding implies an additional late-stage shift in the differentiation of EEC progenitors. Given the limited functional understanding of ZNF800 from previous studies, we conducted a comprehensive analysis of its genome-wide binding activity using chromatin immunoprecipitation followed by sequencing (ChIP-seq). This exploration revealed 3085 genes that are bound by ZNF800, exhibiting a gene ontology enrichment in pathways related to neural and the endocrine-gland development. Notably, key TFs including NEUROG3, PAX4, INSM1 and SOX4 emerged as central players among these downstream targets. Intriguingly, our study demonstrated that only the knockout of PAX4 resulted in the reversal of EEC differentiation, shifting from ECs to other rare EEC subtypes, including L-cells. Furthermore, we unveiled a unilateral inhibition of PAX4 towards ARX, a TF known to be essential for L-, G-, I- and N-cells, indicating PAX4 is responsible for EC lineage specification by suppressing other lineages. Collectively, our downstream analysis provides a mechanistic overview of ZNF800's repressive function in EEC differentiation by directly targeting a network of endocrine TFs (Figure 2C). Our work highlights the utility of optimized human organoids for CRISPR-based functional screens, facilitating the identification of regulators in human gut physiology and pathophysiology. While several co-expression network studies have explored the human endocrine development, ZNF800 has not been discovered through co-expressed gene modules, primarily due to its ubiquitous expression across the human body. Therefore, functional screening in organoid models demonstrates its potency in high-throughput target identification, enabling the unbiased interrogation of the gene function in vitro. Moreover, our study provides mechanistic insights in how the endocrine cell development is controlled by both positive and negative regulators. Given the conserved role of NEUROG3 in the endocrine cell development in both the pancreas and gut, it is a plausible that ZNF800 may regulate endocrine cell differentiation in the human pancreas through the TF network described in this study. Further investigation in human adult pancreas organoids may address this question. Lin Lin: Writing – Original Draft Preparation. Hans Clevers: Writing – Review & Editing. This work was supported by the European Research Council under ERC advanced grant no. 101020405 (GutHormones) (L.L. and H.C.). H.C. is an inventor on patents held by the Royal Netherlands Academy of Arts and Sciences that cover organoid technology. He is currently head of pharma Research and Early Development (pRED) at Roche, Basel, Switzerland. H.C.'s full disclosure is given at https://www.uu.nl/staff/JCClevers/. Not applicable. Not applicable.
In the present study, we examine crossover - the transmission of stress and strain from one spouse to another - in a sample of 78 information technology (IT) professionals and their working spouses. This research places crossover within the role theory framework via work-home interference (WHI) and home-work interference (HWI). The research design was cross-sectional and used self-report data. Results of hierarchical regression analysis indicated the following: (H1) For IT professionals, WHI was directly linked to work-related outcomes (i.e., burnout, health complaints); (H2) Crossover effects were found between the partner HWI and the exhaustion of the IT professional. For IT professionals, negative affectivity was significantly associated with exhaustion and cynicism, whereas for the spouse, negative affectivity was significantly associated with exhaustion and turnover intentions. The relevance of these findings to crossover research is discussed.
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CAGE peak based table with annotated peaks for human samples
No abstract is provided for this article.
The most popular instrument to measure burnout is the Maslach Burnout Inventory (MBI). Recently, to overcome some of the limitations of the MBI, a new instrument has been proposed, namely the Burnout Assessment Tool. The purpose of this study is to examine the psychometric properties of the Italian version of the BAT. This tool is comprised of a set of four core dimensions (BAT-C; i.e., exhaustion, mental distance, cognitive and emotional impairment) and two secondary symptom dimensions (BAT-S; i.e., psychological and psychosomatic complaints). Data were collected on a sample of 738 participants from heterogeneous sectors and professional roles. In the sample women were slightly overrepresented (52.9%), the participants had a mean age of 41.57 years (SD = 10.51) and a mean organizational tenure of 9.65 years (SD = 8.50). The reliability and factorial structure of the BAT-C and BAT-S, together with the convergent and discriminant validity of BAT-C and MBI were explored, as well as the incremental validity to the BAT-C, over and beyond the MBI. Our results confirmed the factorial validity of a two-factor second-order factor model (BAT-C and BAT-S) represented by 4 first-order factors in the case of BAT-C and 2 first-order factors for BAT-S. Results also attested that BAT-C explains additional variance of the BAT-S, above and beyond what is explained by the MBI-GS. All in all, this study provided evidence that the Italian version of BAT represents a reliable and valid tool for measuring burnout in the work context.
Colorectal cancer (CRC) is associated with the presence of particular gut microbes, as observed in many metagenomic studies to date. However, in most cases, it remains difficult to disentangle their active contribution to CRC from just a bystander role. This review focuses on the mechanisms described to date by which the CRC-associated microbiota could contribute to CRC. Bacteria like pks + Escherichia coli, Fusobacterium nucleatum, or enterotoxigenic Bacteroides fragilis have been shown to induce mutagenesis, alter host epithelial signaling pathways, or reshape the tumor immune landscape in several experimental systems. The mechanistic roles of other bacteria, as well as newly identified fungi and viruses that are enriched in CRC, are only starting to be elucidated. Additionally, novel systems like organoids and organs-on-a-chip are emerging as powerful tools to study the direct effect of gut microbiota on healthy or tumor intestinal epithelium. Thus, the expanding knowledge of tumor-microbiota interactions holds promise for improved diagnosis and treatment of CRC.
