<title>Abstract</title> This chapter reviews the effects of water deficits on growth and yield of lentil and explores the genetic and agronomic options to minimize the effects of drought on dry matter (DM) production and seed yields.
We simulated pre-breeding in evolving gene banks - populations of exotic and crop types undergoing optimal contribution selection for long-term genetic gain and management of population genetic diversity. The founder population was based on crosses between elite crop varieties and exotic lines of field pea (Pisum sativum) from the primary genepool, and was subjected to 30 cycles of recurrent selection for an economic index composed of four traits with low heritability: black spot resistance, flowering time and stem strength (measured on single plants), and grain yield (measured on whole plots). We compared a small population with low selection pressure, a large population with high selection pressure, and a large population with moderate selection pressure. Single seed descent was compared with S0-derived recurrent selection. Optimal contribution selection achieved higher index and lower population coancestry than truncation selection, which reached a plateau in index improvement after 40 years in the large population with high selection pressure. With optimal contribution selection, index doubled in 38 years in the small population with low selection pressure and 27-28 years in the large population with moderate selection pressure. Single seed descent increased the rate of improvement in index per cycle but also increased cycle time.
Abstract The dominance of a few staple crops (maize, rice, and wheat) in most agricultural systems hampers the application of interventions to improve food security and nutrition. Research and development attention has focused on improving the production and utilization of these crops, leaving other crops under‐researched and underutilized. Subsequently, there have been high malnutrition rates due to poor diet diversity, yet there are “opportunity crops” that remain under researched. The opportunity crops can unlock solutions to food insecurity, malnutrition, a lack of biodiversity, and indeed poor climate adaptation. The study explored diversification in agricultural systems to analyze whether reorientation of research investment to include under‐researched crops can increase nutrient gain and enhance dietary diversity. Research outputs benchmarked as the number of publications from three leading African universities, Nairobi, Pretoria, and Ghana, were related to crop diversity and nutrition of crops in five clusters: cereals, vegetables, legumes, roots and tubers, and nuts. The findings show that maize was the predominantly researched crop across the three institutions. Low research outputs were observed for pearl millet, finger millet, and yam across the three institutions: amaranth and nightshade (Pretoria), sweet potatoes (Pretoria and Ghana), Marama bean (Nairobi), and soya bean (Nairobi and Ghana). There was nutrient gain across all five clusters, particularly from under‐researched indigenous crops such as finger millet, amaranth, nightshade, yam, sweet potatoes, Marama bean, and soybean. Nutrient gain was contributed more by cereals and root and tuber crops from Pretoria, vegetables and nuts (Ghana), as well as legumes (Nairobi). The findings demonstrate that incorporating research on the least researched crops with successful integration of other research and development initiatives (policy and dissemination) can increase nutrition and improve dietary diversity. The nutrient gain will positively affect food security and nutrition, contributing to the achievement of Africa Agenda 2063, the United Nation's Sustainable Development Goals, and reducing food imports. The findings can inform research investment and decision across different institutions within the African continent. Research investment targeting crops such as finger millet, amaranthus, sweet potatoes, soya beans, and cashew nuts is needed considering the nutritional contribution, climate change adaptability, market potential, and biodiversity contribution. Further analysis should explore production, socio‐economic (marketability and income generation), and environmental gains (adaptive ability to climate change) for specific crops. The development of frameworks to guide the analysis of the nature and scope of factors affecting the contribution of these crops to food security and nutrition, as well as research on specific crops considering geographic distribution and institutional involvement, is also needed.
<title>Abstract</title> Background Weed infestation is one of the major yield-reducing factors in wheat in dry-land farming. Metribuzin is a broad-spectrum herbicide which allow effective weed management in wheat but the narrow safety margin results in crop damage decreasing grain yield. Improving our understanding of the genetic and genomic basis for metribuzin tolerance opens the potential to enhance herbicide tolerance and better productivity in wheat. The present investigation examines the genes involved in regulation of metribuzin tolerance including genetic/signalling pathways, transcription factors, phytohormones, and gene based EST-SSR markers related to photosynthesis and metabolic detoxification. Results Transcriptome sequencing of most diverse genotypes using high throughput NovaSeq 6000 RNA-Seq platform identified a total of 77,443 genes, of which 59,915 were known genes and 17,528 were novel genes. The integrative analyses of the expression profiles of genes and pathways at 0 h, 24 h and 60 h herbicide exposure indicated that modulation of reactive oxygen species (ROS) homeostasis and endogenous increase of light-harvesting chlorophyll (<italic>Lhc)</italic> a/b-binding proteins, PSII stability factor HCF136, metabolic detoxification enzymes (peroxidase, cytochrome P450, glycosyltransferase, glutathione transferase, oxidoreductase), and glucose metabolism conferred metribuzin tolerance in wheat. The validation of DEGs related to photosynthesis (<italic>Lhc a/b-</italic>binding proteins and PSII stability factor HCF136) and metabolic enzymes (cytochrome P450, peroxidase) using RT-qPCR confirmed their responsiveness to metribuzin. Over-expression of transcription factors MYB, AP2-EREBP, ABI3VP1, bHLH, and NAC played a significant roles in regulating photosynthetic and ROS scavenging activities during metribuzin stress. Transcripts with significant enrichments (q-value < 0.05), related to photosynthesis and metabolic detoxification revealed 114 EST-SSRs which may be used as bio-markers. Conclusions The integrative analyses of the data suggests that high amount of sugars, modulation of ROS homeostasis and enhanced photosynthetic activity play a significant role in regulating metribuzin tolerance in wheat. Our data identified master regulators controlling metribuzin tolerance that provide promising avenues for wheat industry.