Drought and heat in dryland agriculture challenge the enhancement of crop productivity and threaten global food security. This review is centered on harnessing genetic variation through biotechnology-led approaches to select for increased productivity and stress tolerance that will enhance crop adaptation in dryland environments. Peer-reviewed literature, mostly from the last decade and involving experiments with at least two seasons' data, form the basis of this review. It begins by highlighting the adverse impact of the increasing intensity and duration of drought and heat stress due to global warming on crop productivity and its impact on food and nutritional security in dryland environments. This is followed by (1) an overview of the physiological and molecular basis of plant adaptation to elevated CO<sub>2</sub> (eCO<sub>2</sub>), drought, and heat stress; (2) the critical role of high-throughput phenotyping platforms to study phenomes and genomes to increase breeding efficiency; (3) opportunities to enhance stress tolerance and productivity in food crops (cereals and grain legumes) by deploying biotechnology-led approaches [pyramiding quantitative trait loci (QTL), genomic selection, marker-assisted recurrent selection, epigenetic variation, genome editing, and transgene) and inducing flowering independent of environmental clues to match the length of growing season; (4) opportunities to increase productivity in C<sub>3</sub> crops by harnessing novel variations (genes and network) in crops' (C<sub>3</sub>, C<sub>4</sub>) germplasm pools associated with increased photosynthesis; and (5) the adoption, impact, risk assessment, and enabling policy environments to scale up the adoption of seed-technology to enhance food and nutritional security. This synthesis of technological innovations and insights in seed-based technology offers crop genetic enhancers further opportunities to increase crop productivity in dryland environments.
Abstract Drought and cadmium (Cd) stress threaten sustainable crop production, highlighting the need for resilent agricultural practices. Individual application of biochar (BC) and brassinosteroids (24-epibrassinolide; EBL) can mitigate drought and Cd stress. However, their synergistic effects on alleviating drought and Cd stress at transcriptomic level in wheat ( Triticum aestivum L.) remain underexplored. This study investigated the combined impact of BC (B 0 = 0% w/w and B 1 = 5% w/w) and EBL (H 0 = control and H 1 = 10 –6 M) on wheat physiology, biomass, and digital gene expression under Cd (30 ppm) and drought stress (D 0 = 75% water holding capacity (WHC) and D 1 = 35% WHC). Drought and Cd stress significantly reduced biomass and photosynthetic activity while increasing oxidative stress and Cd uptake. However, the combined application of BC and EBL treatments showed notable improvements: root fresh biomass, leaf area, and shoot fresh biomass were increased by 39.41%, 66.49%, and 78.25% under D 0 and by 48.24%, 63.76%, and 73.49% under D 1 , respectively, compared to the control. Moreover, Cd uptake by wheat leaves decreased by 71.42% under D 0 and 184.10% under D 1 with BC and EBL combined application. Transcriptome analysis identified 6,174 differentially expressed genes linked to detoxification, carbon and nitrogen metabolism, and stress responses. Gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses highlighted metabolic processes and catalytic functions. Weighted gene co-expression network analysis revealed key modules for stress adaptation, including secondary metabolite biosynthesis and signaling pathways. Transcription factor profiling showed upregulation of AP2/ERF , MYB , and WRKY families in the combined BC and EBL treatments. qPCR validation of RNA-seq data confirmed significant changes in gene expression, with the nitrate transporter and photosystem II CP47 exhibiting increased expression levels by 53.60% and 29.66%, respectively, under BC + EBL treatment at optimal moisture, and 53.38% and 48.82% under drought stress. In contrast, heavy metal transporter genes PMPCB and YCF1 were downregulated, which correlated with a reduction in Cd uptake. Interestingly, the regression analysis demonstrated that Cd concentration in leaves negatively correlated with ( dehydrin-/LEA group ) and ( cadmium tolerance factor ). Overall, this study confirms that combining BC and EBL effectively mitigates Cd stress in drought-affected wheat, enhancing growth and resilience. Graphical abstract
Despite advancements in electrification and the transition to solar-based electricity production, India will continue to depend on land-based carbon offsets to achieve its net-zero target. Land-based climate mitigation strategies in India can be implemented by utilizing underutilized marginal lands or increasing land availability through technological interventions to close agricultural yield gaps. Both below-ground (e.g., soil carbon) and above-ground (e.g., standing tree biomass) options offer viable pathways for such measures. Key strategies include cultivating perennial bioenergy feedstocks, afforestation, establishing fast-growing Miyawaki forests, restoring wetlands and mangroves, and applying biosolids to land. However, caution is essential to prevent unintended consequences, such as clearing natural forests or introducing microplastics into soils. The cost of carbon sequestration and the resilience or permanence of stored carbon will be critical factors in determining the preferred approach. Additionally, land-based strategies often overlap spatially, making GIS-based tools indispensable for identifying optimal solutions tailored to local conditions. Integrating these strategies into the national carbon budget can enhance transparency and contribute significantly to India’s net-zero emissions goal.
Reproductive processes of chickpea (Cicer arietinum L.) are particularly sensitive to salinity. We tested whether limited photoassimilate availability contributes to reproductive failure in salt-stressed chickpea. Rupali, a salt-sensitive genotype, was grown in aerated nutrient solution, either with non-saline (control) or 30mM NaCl treatment. At flowering, stems were either infused with sucrose solution (0.44M), water only or maintained without any infusion, for 75 d. The sucrose and water infusion treatments of non-saline plants had no effect on growth or yield, but photosynthesis declined in response to sucrose infusion. Salt stress reduced photosynthesis, decreased tissue sugars by 22-47%, and vegetative and reproductive growth were severely impaired. Sucrose infusion of salt-treated plants increased total sugars in stems, leaves and developing pods, to levels similar to those of non-saline plants. In salt-stressed plants, sucrose infusion increased dry mass (2.6-fold), pod numbers (3.8-fold), seed numbers (6.5-fold) and seed yield (10.4-fold), yet vegetative growth and reproductive failure were not rescued completely by sucrose infusion. Sucrose infusion partly rescued reproductive failure in chickpea by increasing vegetative growth enabling more flower production and by providing sucrose for pod and seed growth. We conclude that insufficient assimilate availability limits yield in salt-stressed chickpea.