Publications

The role of NF-κB activation in tumor initiation has not been thoroughly investigated. We generated Ikkβ(EE) IEC transgenic mice expressing constitutively active IκB kinase β (IKKβ) in intestinal epithelial cells (IECs). Despite absence of destructive colonic inflammation, Ikkβ(EE) IEC mice developed intestinal tumors after a long latency. However, when crossed to mice with IEC-specific allelic deletion of the adenomatous polyposis coli ( Apc ) tumor suppressor locus, Ikkβ(EE) IEC mice exhibited more β-catenin + early lesions and visible small intestinal and colonic tumors relative to Apc +/ΔIEC mice, and their survival was severely compromised. IEC of Ikkβ(EE) IEC mice expressed high amounts of inducible nitric oxide synthase (iNOS) and elevated DNA damage markers and contained more oxidative DNA lesions. Treatment of Ikkβ(EE) IEC / Apc +/ΔIEC mice with an iNOS inhibitor decreased DNA damage markers and reduced early β-catenin + lesions and tumor load. The results suggest that persistent NF-κB activation in IEC may accelerate loss of heterozygocity by enhancing nitrosative DNA damage.

<p>Supplementary Methods</p>

Neighborhood-level socioeconomic deprivation can increase risk for higher blood pressure or hypertension, while greater neighborhood safety and walkability may protect against hypertension. Large-scale prospective research, particularly among Hispanics/Latinos, is lacking. We examined cross-sectional and prospective associations between neighborhood environments and blood pressure and hypertension among 3851 Hispanic/Latinos enrolled in the Hispanic Community Health Study/Study of Latinos San Diego, CA cohort. Addresses from Visit 1 (2008–2011) were geocoded and neighborhood characteristics were determined as part of the SOL CASAS ancillary study. Home addresses were geocoded and home areas created using 800 m circular radial buffers. Neighborhood indices socioeconomic deprivation, residential stability, and social disorder were created using Census and other publicly available data. Walkability was computed as density of intersections, retail spaces, and residences. Greenness was measured via satellite imagery using the Normalized Difference Vegetation Index. Visit 1 and Visit 2 (2014–2017) clinical outcomes included systolic (SBP) and diastolic (DBP) blood pressure, as well as prevalent and 6-year incident hypertension, defined as SBP/DBP ≥140/90 mmHg or antihypertensive medication use. Complex survey regression models adjusted for covariates revealed cross-sectional associations between greater walkability and lower SBP (B = −0.05; 95% CI: −0.09, −0.003). In prospective analyses, greater neighborhood social disorder was related to increasing SBP (B = 0.05; 95% CI: 0.01, 0.09) and DBP (B = 0.07; 95% CI: 0.02, 0.12) over time. Greater socioeconomic deprivation (OR = 1.47; 95% CI: 1.06, 2.04) and greater social disorder (OR = 1.25; 95% CI: 1.02, 1.54) were associated with higher odds of incident hypertension. All other associations were not significant. Beyond individual-level characteristics, greater neighborhood social disorder and socioeconomic deprivation were related to adverse changes in blood pressure over 6 years among Hispanics/Latinos. Neighborhood social environment may help identify, or be an area for future intervention for, cardiovascular risk among Hispanics/Latinos.

Park use is associated with health, yet our understanding of park features related to their use is limited. Singapore's parks were audited for 30 micro-features, then geospatial analysis characterized micro-features scores for parks nearest to participants' homes. Adults (3,435) reported their park use and park-based physical activity. Using linear regression models, we found living near a park with higher micro-features scores was associated with more time in parks and park-based physical activity. Specific micro-features were associated with more park time (wildlife areas, water features, forested areas, unpaved trails (2–2.6 h/month, p < 0.05)) and with physical activity in parks (water features, forested areas, large playground, open green spaces (1.8–2.2 h/month, p < 0.05)). These findings could inform parks planning to support population-health.

This study examined youth physical activity (PA), correlates of parental rules about children's PA, perceived barriers, and use of PA facilities by neighborhood walkability and median household income. Parents (N 517) from 32 neighborhoods completed a questionnaire related to the PA of their youngest or only child aged 4–18 years living in the household. Using mixed regression analysis, results showed that neighborhood income and walkability played a significant role in parental perceptions of barriers and their rules for allowing their children to participate in physical activities. Interventions to promote children's PA may need to vary by neighborhood characteristics.

Project S.H.O.U.T. (which stands for “Students Helping Others Understand Tobacco”) is a tobacco-use prevention project funded by the United Sta

Data from a randomized clinical trial were used to examine the extent to which a health promotion intervention affected changes in psychosocial constructs and if so whether these in turn explained changes in physical activity (PA). PA and psychosocial data on 878 adolescents (ages 11—15) recruited through primary care providers (age M = 12.7 years, SD = 1.3; 58% white non-Hispanic) were measured at baseline, six and 12 months. Parallel process latent growth curve analyses found positive relationships between the growth trajectories of behavior change strategies, self-efficacy, family support, peer support and the growth trajectory of PA. However, mediation analyses did not reveal statistically significant intervention-mediated effects.

There is growing evidence that communities can be designed to support physical activity, but it is important to understand whether neighborhood features related to health are also considered satisfactory by residents. The study aimed to determine if there is an association between perceived and objective neighborhood environment variables and neighborhood satisfaction. Adults ( N = 1,726) were recruited from neighborhoods in two regions of the United States selected to vary on walkability and income. Perceived neighborhood environment was assessed using a validated scale, objective measures were constructed using geographic information system (GIS), and satisfaction was assessed using a 17-item survey. Participants reported greater satisfaction when they perceived their neighborhood as having greater pedestrian/traffic safety, crime safety, attractive aesthetics, access to destinations, diversity of destinations, park access, and lower residential density. Objective measures were not significant. The discrepant findings between perceived and objective environmental measures indicate that neighborhood satisfaction is a complex construct.