Publications

Current distributed routing paradigms (such as link-state, distance-vector, and path-vector) involve a convergence process consisting of an iterative exploration of intermediate routes triggered by certain events such as link failures. The convergence process increases router load, introduces outages and transient loops, and slows reaction to failures. We propose a new routing paradigm where the goal is not to reduce the convergence times but rather to eliminate the convergence process completely. To this end, we propose a technique called Failure-Carrying Packets (FCP) that allows data packets to autonomously discover a working path without requiring completely up-to-date state in routers. Our simulations, performed using real-world failure traces and Rocketfuel topologies, show that: (a) the overhead of FCP is very low, (b) unlike traditional link-state routing (such as OSPF), FCP can provide both low loss-rate as well as low control overhead, (c) compared to prior work in backup path pre-computations, FCP provides better routing guarantees under failures despite maintaining lesser state at the routers.

Abstract Shewanella spp. are a group of facultative anaerobic bacteria widely distributed in marine and freshwater environments. In this study, we profiled the central metabolic fluxes of eight recently sequenced Shewanella species grown under the same condition in minimal medium with [3‐ 13 C] lactate. Although the tested Shewanella species had slightly different growth rates (0.23–0.29 h −1 ) and produced different amounts of acetate and pyruvate during early exponential growth (pseudo‐steady state), the relative intracellular metabolic flux distributions were remarkably similar. This result indicates that Shewanella species share similar regulation in regard to central carbon metabolic fluxes under steady growth conditions: the maintenance of metabolic robustness is not only evident in a single species under genetic perturbations (Fischer and Sauer, 2005; Nat Genet 37(6):636–640), but also observed through evolutionary related microbial species. This remarkable conservation of relative flux profiles through phylogenetic differences prompts us to introduce the concept of metabotype as an alternative scheme to classify microbial fluxomics. On the other hand, Shewanella spp. display flexibility in the relative flux profiles when switching their metabolism from consuming lactate to consuming pyruvate and acetate. Biotechnol. Bioeng. 2009;102: 1161–1169. © 2008 Wiley Periodicals, Inc.

The interaction of the metal and support in oxide-supported transition-metal catalysts has been proven to have extremely favorable effects on catalytic performance. Herein, mesoporous Co3O4, NiO, MnO2, Fe2O3, and CeO2 were synthesized and utilized in CO oxidation reactions to compare the catalytic activities before and after loading of 2.5 nm Pt nanoparticles. Turnover frequencies (TOFs) of pure mesoporous oxides were 0.0002–0.015 s–1, while mesoporous silica was catalytically inactive in CO oxidation. When Pt nanoparticles were loaded onto the oxides, the TOFs of the Pt/metal oxide systems (0.1–500 s–1) were orders of magnitude greater than those of the pure oxides or the silica-supported Pt nanoparticles. The catalytic activities of various Pt/oxide systems were further influenced by varying the ratio of CO and O2 in the reactant gas feed, which provided insight into the mechanism of the observed support effect. In situ characterization using near-edge X-ray absorption fine structure (NEXAFS) and ambient-pressure X-ray photoelectron spectroscopy (APXPS) under catalytically relevant reaction conditions demonstrated a strong correlation between the oxidation state of the oxide support and the catalytic activity at the oxide–metal interface. Through catalytic activity measurements and in situ X-ray spectroscopic probes, CoO, Mn3O4, and CeO2 have been identified as the active surface phases of the oxide at the interface with Pt nanoparticles.

Abstract Cuprous copper (Cu(I)) is an essential cofactor for enzymes supporting many cellular functions including mitochondrial respiration and suppression of oxidative stress. Neurons are particularly dependent on these pathways, with multiple neurodegenerative diseases, including Alzheimer’s disease (AD), Parkinson’s disease, associated with their dysfunction. Key features of Cu(I) contributions to neuronal health in vivo remain to be defined, owing largely to the complex processes involved in Cu(I) production, intracellular transport, and systemic redistribution. Here, we provide genetic and pharmacological evidence that swip-10 is a critical determinant of systemic Cu(I) levels in C. elegans , with deletion leading to systemic deficits in mitochondrial respiration, production of oxidative stress, and neurodegeneration. These phenotypes can be reproduced in wild-type worms by Cu(I)-specific chelation and offset in swip-10 mutants by growth on the Cu(I) enhancing molecule elesclomol, as well as by glial expression of wildtype swip-10 . MBLAC1 , the most closely related mammalian ortholog to swip-10 , encodes for a pre-mRNA processing enzyme for H3 histone, a protein whose actions surprisingly include an enzymatic capacity to produce Cu(I) via the reduction of Cu(II). Moreover, genome-wide association studies and post-mortem molecular studies implicate reductions of MBLAC1 expression in risk for AD with cardiovascular disease comorbidity. Consistent with these studies, we demonstrate that the deposition of β-amyloid plaques, an AD pathological hallmark, in worms engineered to express human Aβ 1-42, is greatly exaggerated by mutation of swip-10 . Together, these studies identify a novel glial-expressed, and pathway for Cu(I) production that may be targeted for the treatment of AD and other neurodegenerative diseases. Significance Statement Devastating neurodegenerative diseases such as Alzheimer’s disease, and Parkinson’s disease are associated with disruptions in copper (Cu) homeostasis. Alterations in Cu(I) give rise to increased oxidative stress burden, mitochondrial and metabolic dysfunction, and can accelerate production and/or potentiate toxicity of disease-associated protein aggregates. Here, using the model system Caenorhabditis elegans , we establish a role for the gene swip-10 in systemic Cu(I) homeostasis. Perturbation of this pathway in worms recapitulates biochemical, histological, and pathological features seen in human neurodegenerative disease. We reveal that these changes can be suppressed pharmacologically and arise when swip-10 expression is eliminated from glial cells. Our work implicates swip-10 and orthologs as key players in Cu(I) homeostasis that may be exploitable to treat multiple neurodegenerative diseases.

Although the human visual system is surprisingly robust to extreme distortion when recognizing objects, most evaluations of computer object detection methods focus only on robustness to natural form deformations such as people's pose changes. To determine whether algorithms truly mirror the flexibility of human vision, they must be compared against human vision at its limits. For example, in Cubist abstract art, painted objects are distorted by object fragmentation and part-reorganization, to the point that human vision often fails to recognize them. In this paper, we evaluate existing object detection methods on these abstract renditions of objects, comparing human annotators to four state-of-the-art object detectors on a corpus of Picasso paintings. Our results demonstrate that while human perception significantly outperforms current methods, human perception and part-based models exhibit a similarly graceful degradation in object detection performance as the objects become increasingly abstract and fragmented, corroborating the theory of part-based object representation in the brain.

Fatigue resistance is invariably critical for structural materials, but is rarely considered in the development of new bioinspired materials. Here the fatigue behavior and damage mechanisms of a nacre-like ceramic (yttria-stabilized zirconia) - polymer (polymethyl methacrylate) composite, which resembles human tooth enamel in its stiffness and hardness, were investigated under cyclic compression to simulate potential service conditions. The composite has a brick-and-mortar structure which exhibits a staircase-like fracture behavior; it displays a transition in cracking mode from intergranular splitting of ceramic bricks to separation along the inter-brick polymer phase with increasing stress amplitude. The nacre-like structure functions to induce crack deflection, increase the roughness of crack surfaces, and promote the mutual sliding between bricks during fracture; this results in high fatigue resistance, which enhances the potential of this composite for dental applications.