Publications

Abstract Monoterpenes are commonly known for their role in the flavors and fragrances industry and are also gaining attention for other uses like insect repellant and as potential renewable fuels for aviation. Corynebacterium glutamicum, a Generally Recognized as Safe microbe, has been a choice organism in industry for the annual million ton-scale bioproduction of amino acids for more than 50 years; however, efforts to produce monoterpenes in C. glutamicum have remained relatively limited. In this study, we report a further expansion of the C. glutamicum biosynthetic repertoire through the development and optimization of a mevalonate-based monoterpene platform. In the course of our plasmid design iterations, we increased flux through the mevalonate-based bypass pathway, measuring isoprenol production as a proxy for monoterpene precursor abundance and demonstrating the highest reported titers in C. glutamicum to date at nearly 1500 mg/L. Our designs also evaluated the effects of backbone, promoter, and GPP synthase homolog origin on monoterpene product titers. Monoterpene production was further improved by disrupting competing pathways for isoprenoid precursor supply and by implementing a biphasic production system to prevent volatilization. With this platform, we achieved 321.1 mg/L of geranoids, 723.6 mg/L of 1,8-cineole, and 227.8 mg/L of linalool. Furthermore, we determined that C. glutamicum first oxidizes geraniol through an aldehyde intermediate before it is asymmetrically reduced to citronellol. Additionally, we demonstrate that the aldehyde reductase, AdhC, possesses additional substrate promiscuity for acyclic monoterpene aldehydes. Highlights Design of a mevalonate-based monoterpene production platform in C. glutamicum Highest production titers of geranoids, eucalyptol, and linalool reported in C. glutamicum to date Identification of citronellal as an intermediate in the reduction of geraniol to citronellol by C. glutamicum

ABSTRACT We present measurements of the periods, amplitudes, and types of 74 RR Lyrae stars in the globular cluster M15 derived from Nickel 1 m telescope observations conducted at Lick Observatory in 2019 and 2020. Of these RR Lyrae stars, two were previously reported but without a determination of the period. In addition, we identify five Type II Cepheid variable stars for which we report three novel period determinations, and a further 34 stars with uncertain classifications and periods. We discuss the development and subsequent application to our data of a new python package, Period-determination and Identification Pipeline Suite (pips), based on a new adaptive free-form fitting technique to detect the periods of variable stars with a clear treatment of uncertainties.

We present extensive observations and analysis of supernova (SN) 2021dbg, utilizing optical photometry and spectroscopy. For approximately 385 days following the explosion, SN 2021dbg exhibited remarkable luminosity, surpassing most SNe II. This initial high luminosity is potentially attributed to the interaction between the ejected material and the surrounding circumstellar material (CSM), as evidenced by the pronounced interaction signatures observed in its spectra. The subsequent high luminosity is primarily due to the significant $^{56}$Ni ($0.17 \pm 0.05$ M$_{\odot}$) produced in the explosion. Based on the flux of flash emission lines detected in the initial spectra, we estimate that the CSM mass near the progenitor amounted to $\sim$(1.0--2.0) $\times 10^{-3}$ M$_{\odot}$, likely resulting from intense stellar wind activity 2--3 yr preceding the explosion. Considering the bolometric light curve, nebular spectrum modeling, and mass-loss rate, we suggest that the progenitor of SN 2021dbg was a red supergiant (RSG) with a mass of $\sim 20$ M$_{\odot}$ and a radius of 1200 R$_{\odot}$. This RSG featured a thick hydrogen shell, which may have contained a region with a sharp decrease in material density, electron density, and temperature, contributing to its layered structure. This object demonstrates mixed features of SNe IIP and SNe IIL, making it as a transitional event linking the above two subclasses of SNe II.

Abstract The synthesis of a single‐layer covalent organic framework (COF) with spatially modulated internal potentials provides new opportunities for manipulating the electronic structure of molecularly defined materials. Here, the fabrication and electronic characterization of COF‐420: a single‐layer porphyrin‐based square‐lattice COF containing a periodic array of oriented, type II electronic heterojunctions is reported. In contrast to previous donor–acceptor COFs, COF‐420 is constructed from building blocks that yield identical cores upon reticulation, but that are bridged by electrically asymmetric linkers supporting oriented electronic dipoles. Scanning tunneling spectroscopy reveals staggered gap (type II) band alignment between adjacent molecular cores in COF‐420, in agreement with first‐principles calculations. Hirshfeld charge analysis indicates that dipole fields from oriented imine linkages within COF‐420 are the main cause of the staggered electronic structure in this square grid of atomically–precise heterojunctions.

We report measurements of the mass density, Omega_M, and cosmological-constant energy density, Omega_Lambda, of the universe based on the analysis of 42 Type Ia supernovae discovered by the Supernova Cosmology Project. The magnitude-redshift data for these SNe, at redshifts between 0.18 and 0.83, are fit jointly with a set of SNe from the Calan/Tololo Supernova Survey, at redshifts below 0.1, to yield values for the cosmological parameters. All SN peak magnitudes are standardized using a SN Ia lightcurve width-luminosity relation. The measurement yields a joint probability distribution of the cosmological parameters that is approximated by the relation 0.8 Omega_M - 0.6 Omega_Lambda ~= -0.2 +/- 0.1 in the region of interest (Omega_M 0) = 99%, including the identified systematic uncertainties. The best-fit age of the universe relative to the Hubble time is t_0 = 14.9{+1.4,-1.1} (0.63/h) Gyr for a flat cosmology. The size of our sample allows us to perform a variety of statistical tests to check for possible systematic errors and biases. We find no significant differences in either the host reddening distribution or Malmquist bias between the low-redshift Calan/Tololo sample and our high-redshift sample. The conclusions are robust whether or not a width-luminosity relation is used to standardize the SN peak magnitudes.

An interactive robot framework accomplishes long-horizon task planning and can easily generalize to new goals and distinct tasks, even during execution. However, most traditional methods require predefined module design, making it hard to generalize to different goals. Recent large language model based approaches can allow for more open-ended planning but often require heavy prompt engineering or domain specific pretrained models. To tackle this, we propose a simple framework that achieves interactive task planning with language models by incorporating both high-level planning and low-level skill execution through function calling, leveraging pretrained vision models to ground the scene in language. We verify the robustness of our system on the real world task of making milk tea drinks. Our system is able to generate novel high-level instructions for unseen objectives and successfully accomplishes user tasks. Furthermore, when the user sends a new request, our system is able to replan accordingly with precision based on the new request, task guidelines and previously executed steps. Our approach is easy to adapt to different tasks by simply substituting the task guidelines, without the need for additional complex prompt engineering. Please check more details on our https://wuphilipp.github.io/itp_site and https://youtu.be/TrKLuyv26_g.

Extracting 3D shape of deforming objects in monocular videos, a task known as non-rigid structure-from-motion (NRSfM), has so far been studied only on syn-thetic datasets and controlled environments. Typically, the objects to reconstruct are pre-segmented, they exhibit limited rotations and occlusions, or full-length trajectories are assumed. In order to integrate NRSfM into current video analy-sis pipelines, one needs to consider as input realistic-thus incomplete- tracking, and perform spatio-temporal grouping to segment the objects from their surround-ings. Furthermore, NRSfM needs to be robust to noise in both segmentation and tracking, e.g., drifting, segmentation “leaking”, optical flow “bleeding ” etc. In this paper, we make a first attempt towards this goal, and propose a method that combines dense optical flow tracking, motion trajectory clustering and NRSfM for 3D reconstruction of objects in videos. For each trajectory cluster, we com-pute multiple reconstructions by minimizing the reprojection error and the rank of the 3D shape under different rank bounds of the trajectory matrix. We show that dense 3D shape is extracted and trajectories are completed across occlusions and low textured regions, even under mild relative motion between the object and the camera. We achieve competitive results on a public NRSfM benchmark while using fixed parameters across all sequences and handling incomplete trajectories, in contrast to existing approaches. We further test our approach on popular video segmentation datasets. To the best of our knowledge, our method is the first to extract dense object models from realistic videos, such as those found in Youtube or Hollywood movies, without object-specific priors. 1

A mechanistic study on the origin of the difference in reactivity between Ir catalysts for C-H borylation reactions is reported. Catalytic reactions of B₂pin₂ with a series of substrates that require high temperatures and long reaction times were conducted. These reactions catalyzed by the combination of [Ir(COD)(OMe)]₂ and 3,4,7,8-tetramethylphenanthroline (tmphen) occur in yields that are substantially higher than those of reactions catalyzed by [Ir(COD)(OMe)]₂ and 4,4'-di-<i>tert</i>-butylbipyridine (dtbpy). The electronic properties of Ir catalysts ligated by dtbpy or tmphen and their stoichiometric reactivity were investigated. It was found that a longer lifetime rather than higher reactivity of the catalyst leads to higher yields of reactions catalyzed by Ir-tmphen. The catalyst ligated by dtbpy decomposes principally by dissociation of the ligand and rapid borylation at the positions alpha to nitrogen. Thus, the greater stability of the catalyst containing tmphen results from its greater binding constant.