Publications

Therapeutic potential of 9-(2-phosphonylmethoxyethyl)adenine (PMEA) as an antiviral drug

[Penetration and action of edoxudine in vitro and in vivo].

The penetration of 5-ethyl-2'-deoxyuridine (edoxudine, Aedurid) from gel base with and without the addition of urea and other adjuvant has been studied in an in vitro model using guinea pig skin. The formulation of 3% edoxudine gel with 5% urea showed the best results. In vivo experiments on hairless mice infected intracutaneously with herpes simplex virus type 1 also showed this formulation's good efficacy as compared to other formulations.

The synthesis and properties of some 5-substituted uracil derivatives.

The chemical syntheses of some 5-substituted uracil deorivatives, in particular 5-vinyl-2'-deoxyuridine, 5-ethynyl-2'-deoxyuridine and 4-(2-bromovinyl)-2'-deoxy-uridine are reviewed and their potential as radiation sensitizing agents, anti-cancer agents and antiviral agents is discussed. 5-Ethynyl-2'-deoxyuridine is not incorporated into DNA; is a thymidylate synthetase inhibitor and has a possible use as an anti-cancer drug. 5-Vinyl-2'-dexyuridine can replace thymidine residues in DNA of phage T3; does not cause the organism to be significantly more sensitive to gamma-radiation but its presence in DNA causes the organisms to be significantly more sensitive to gamma-radiation but its presence in DNA causes the organism to lose viability, possibly through chemical cross-linking reactions of the vinyl group. 5-(2-Bromovinyl)-2'-deoxyuridine is the most specific and potent antiherpes compound yet known. Its mode of action and its affects on herpes virus in vitro and in vivo with animal models and clinical observations are described.

Lithiation of Uracilnucleosides and its Application to the Synthesis of a New Class of Anti-HIV-1 Acyclonucleosides

Abstract A highly general and regioselective modification of the base moiety of uracilnucleosides can be accomplished based on lithiation chemistry. An application of this approach to the synthesis of various acyclouridine derivatives, in which both C-5 and C-6 positions were substituted, was carried out to improve the HIV-1 specific inhibitory activity of a new lead compound, l-[(2-hydroxyethoxy)methyl]-6-phenylthiothymine (HEPT).

Differential inhibition of various deoxyribonucleic acid polymerases by Evans blue and aurintricarboxylic acid

CCDC 192243: Experimental Crystal Structure Determination

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

Self-calibration method and software error correction for three-dimensional coordinate measuring machines using artefact measurements

The proposed self-calibration method uses an artefact, a ball plate, for error identification. Unlike other methods this ball plate need not be calibrated, i.e. the distances between the different balls on the plate need not be known accurately. After measurement of the ball plate in different positions within the measuring volume, the systematic geometrical errors, like scale errors, axes' pitch, yaw and non-orthogonality, of the geometric error model of the measuring machine can be calculated. This geometrical error model is used afterwards by the software error correction to correct on-line every single measurement carried by the measuring machine. This results in a higher accuracy of the measurement. As an example, the self-calibration method has been carried out on a CNC 3-D coordinate measuring machine and the resulting software error correction has been evaluated using step-gauge measurements. The results of the evaluation show that the systematic geometrical errors are greatly reduced.

CCDC 891844: Experimental Crystal Structure Determination

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

Mannose-specific plant lectins as potential HIV microbicides

ChemInform Abstract: Synthesis, Lipophilicity and Biological Properties of Some Novel 1H‐1,2,4 Triazole Derivatives.

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.

Manufacturing of µIM Mould Inserts with AMed Cooling Channels

No abstract is provided for this article.

Synthesis of new 6-substituted purinyl-5′-nor-1′-homocarbanucleosides based on indanol

Dihydro-alkoxyl-benzyl-oxopyrimidine Derivatives (DABOs) As Non-Nucleoside Reverse Transcriptase Inhibitors: An Update Review (2001-2011)

Numerous structurally different non-nucleoside compounds have been evaluated for their inhibitory effects against HIV replication, in which DABO derivatives, bearing the dihydro-alkoxyl-benzyl-oxopyrimidine scaffold, have been identified as a particular class of non-nucleoside reverse transcriptase inhibitors (NNRTIs). The S-DABO, NH-DABO, -N-DABO, DATNO and DACO analogs represent various structural chemical modifications of the substituents on C2, C6 and C5 of the pyrimidine ring to obtain potentially higher potency and lower cytotoxicity. This review article describes the recent progress of the chemical modifications and structure-activity relationship studies of DABO derivatives and provides new clues for the design of new DABO congeners.

ChemInform Abstract: Pyridazines. Part 92. Synthesis of Dialkyldipyridazinodiazepinones as Potential HIV‐1 Reverse Transcriptase Inhibitors.

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.

5-Cyano substituted diarylpyridines as potent HIV-1 NNRTIs: Rational design, synthesis, and activity evaluation

Antiviral and Non-antiviral Activity of Highly Purified Interferon

Detection of immediate early, early and late antigens of human cytomegalovirus by flow cytometry

Discovery of Novel Aryl Triazolone Dihydropyridines (ATDPs) Targeting Highly Conserved Residue W229 as Promising HIV-1 NNRTIs

NNRTI is an important component of the highly active antiretroviral therapy (HAART), but the rapid emergence of drug resistance and poor pharmacokinetics limited their clinical application. Herein, a series of novel aryl triazolone dihydropyridines (ATDPs) were designed by structure-guided design with the aim of improving drug resistance profiles and pharmacokinetic profiles. Compound <b>10n</b> (EC₅₀ = 0.009-17.7 μM) exhibited the most active potency, being superior to or comparable to that of doravirine (DOR) against the whole tested viral panel. Molecular docking was performed to clarify the reason for its higher resistance profiles. Moreover, <b>10n</b> demonstrated excellent pharmacokinetic profile (<i>T</i>_1/2 = 5.09 h, <i>F</i> = 108.96%) compared that of DOR (<i>T</i>_1/2 = 4.4 h, <i>F</i> = 57%). Additionally, <b>10n</b> was also verified to have no <i>in vivo</i> acute or subacute toxicity (LD₅₀ > 2000 mg/kg), suggesting that <b>10n</b> is worth further investigation as a novel oral NNRTIs for HIV-1 therapy.