Abstract This work compares the denaturation of two proteins—bovine carbonic anhydrase II (BCA) and its derivative with all lysine groups acetylated (BCA‐Ac 18 )—by urea, guanidinium chloride (GuHCl), heat, and sodium dodecyl sulfate (SDS). It demonstrates that increasing the net negative charge of the protein by acetylation of lysines reduces its stability to urea, GuHCl, and heat, but increases its kinetic stability (its thermodynamic stability cannot be measured) towards denaturation by SDS. Increasing the ionic strength of the buffer improves the stability of BCA‐Ac 18 to urea and heat, but still leaves it less stable than unacetylated BCA to those denaturants. In urea, the large change in electrostatic interactions not only modifies the free energy of denaturation, but also introduces a stable intermediate into the unfolding pathway. This work shows that modifications of charges on the surfaces of proteins can have a large effect—positive or negative, depending on the denaturant—on the stability of the proteins despite the exposure of these charges to high dielectric solvent and buffer ions.
This work describes the development of magnetic levitation (MagLev) using ring magnets and a configuration (which we call "axial MagLev") to remove the physical barriers to physical sampling in the magnetic field present in "standard MagLev" and to simplify the procedures used to carry out density-based analyses, separations, and manipulations. The optimized, linear magnetic field generated between the two ring magnets (coaxially aligned and like-poles facing) enables the levitation of diamagnetic (and weakly paramagnetic, e.g., aluminum) materials in a paramagnetic suspending medium and makes density measurements more straightforward. This "axial" configuration enables (i) simple procedures to add samples and paramagnetic medium from an open end and to retrieve samples while levitating in the magnetic field (e.g., a subpopulation of a cluster of small particles); (ii) simple accesses and the abilities to view the samples 360° around the sample container and from the top and bottom; and (iii) convenient density measurements of small quantities (as small as a single submillimeter particle as demonstrated) of samples. The compact design, portability, affordability, and simplicity in use of the "axial MagLev" device will broaden the uses of magnetic methods in analyzing, separating, and manipulating different types of samples (solids, liquids, powders, pastes, gels, and also biological entities) in areas such as materials sciences, chemistry, and biochemistry.
A thin metal film vapor deposited on thick elastomer substrate develops an equi-biaxial compressive stress state when the system is cooled due to the large thermal expansion mismatch between the elastomer and the metal. At a critical stress, the film undergoes buckling into a family of modes with short wavelengths characteristic of a thin plate on a compliant elastic foundation. As the system is further cooled, a highly ordered herringbone pattern has been observed to develop. Here it is shown that the herringbone mode constitutes a minimum energy configuration among a limited set of competing modes.
Gestational diabetes was positively associated with CVD later in life, although the absolute rate of CVD in this younger cohort of predominantly white women was low. This relationship is possibly mediated in part by subsequent weight gain and lack of healthy lifestyle.
No abstract is provided for this article.
The goal of this study is to estimate the thermal impact of a titanium skull unit (SU) implanted on the exterior aspect of the human skull. We envision this unit to house the front-end of a fully implantable electrocorticogram (ECoG)-based bi-directional (BD) brain-computer interface (BCI). Starting from the bio-heat transfer equation with physiologically and anatomically constrained tissue parameters, we used the finite element method (FEM) implemented in COMSOL to build a computational model of the SU's thermal impact. Based on our simulations, we predicted that the SU could consume up to 75 mW of power without raising the temperature of surrounding tissues above the safe limits (increase in temperature of 1°C). This power budget by far exceeds the power consumption of our front-end prototypes, suggesting that this design can sustain the SU's ability to record ECoG signals and deliver cortical stimulation. These predictions will be used to further refine the existing SU design and inform the design of future SU prototypes.
The composition, growth mechanism, and phases of carbon nitride thin films obtained from the reaction of laser ablated carbon and atomic nitrogen have been investigated. The nitrogen composition was found to increase to a limiting value of 50% as the fluence was decreased for laser ablation at both 532 nm and 248 nm. Analysis of these data shows that the overall growth rate determines the nitrogen composition, and suggests that a surface reaction between carbon and nitrogen represents a key step in the growth mechanism. Infrared spectroscopy has also been used to assess the phases present in the carbon nitride thin films. The implications of these results to the stoichiometry of covalent carbon nitride are discussed.
An elastic-plastic analysis is performed for two problems relevant to fracture mechanics: a semiinfinite body with an edge crack in a far out-of-plane shearing field and an infinite plate under plane stress conditions containing a finite line crack in a remote tensile field. Amplitudes of the dominant singularity in the plastic region at the crack tip, the plastic stress and strain intensity factors, are calculated for applied stress levels approaching the yield stress. A technique is developed for using the dominant singular solution in conjunction with the finite element method to make accurate calculations for the near-tip fields. Additionally, a comparative study of deformation theory with flow theory is performed for cracks in an anti-plane shear field. Elastic fracture mechanics is extended to high levels of applied stress for which the plastic zone is no longer small compared to the crack length by relating the critical stress for fracture initiation to the plastic intensity factors.
