<p>Histologic expression of basal markers in HR-positive breast tumors at different tumor stages. <b>A,</b> Representative sections of HR-positive samples at different tumor stages (LGDCIS, DCIS, and IDC) stained with the indicated basal markers. Scale bar, 50 μm. <b>B,</b> Bar plots representing the percentage of cells expressing each marker in in HR-positive samples across the different tumor stages. Statistics calculated two-way ANOVA. *, <i>P</i> < 0.05; **, <i>P</i> < 0.005; ns, not significant.</p>

<p>Primary antibodies used for immunohistochemistry</p>

<p>TAGL as a predictive marker for dasatinib sensitivity. <b>A,</b> Western blot of control and <i>TAGLN</i>-KO cells in Hs578T (top) and BT-549 (bottom) cells. <b>B,</b> Representative immunofluorescence of Hs578T and BT549 control and <i>TAGLN</i>-KO cells, stained with αTAGL (green) and DAPI (blue). Scale bar, 50 μm. <b>C,</b> Cell proliferation in control (black) and <i>TAGLN</i>-KO (purple) cells measured by MTT assays over 72 hours. <b>D,</b> Relative percentage of migrated cells in control and <i>TAGLN</i>-KO cells, assessed by transwell assays. In <b>C</b> and <b>D</b>, values represent the mean ± SEM of at least three experimental replicates, and statistical significance was determined using one-way ANOVA. <b>E,</b> Drug–response curves for cell viability of control and <i>TAGLN</i>-KO cells treated with dasatinib at increasing concentrations. <b>F,</b> Western blot of known dasatinib targets in control and <i>TAGLN</i>-KO cells. <b>G,</b> Data from the <i>DepMap</i> portal showing the correlation between <i>TAGLN</i> and <i>PDGFRB</i> in breast cancer cell lines. <b>H,</b> Western blot of PDGFRβ in <i>TAGLN</i>-KO and cells and clones constitutively expressing <i>PDGFRB</i>. <b>I,</b> Drug–response curves for cell viability of <i>TAGLN</i>-KO and <i>TAGLN</i>-KO/<i>PBGFRB</i> cells treated with dasatinib at increasing concentrations. In <b>E</b> and <b>I</b>, solid lines represent the mean of three biological replicates performed in technical replicates. The dashed line indicates their IC<sub>50</sub> value. **, <i>P</i> < 0.01; ****, <i>P</i> < 0.0001; ns, not significant.</p>

With a steep rise in the urban population requiring an increased number of buildings, public and private transport systems, urban noise is posing a serious environmental problem affecting health. To attenuate the effect on the well-being of human health, a variety of conventional sound-absorbing materials suitable at mid and higher-frequency noise absorption are commonly being used but low and mid-frequency noise remains a challenge. These applications are further limited by the acoustic performance and ventilation efficiency in conventional noise barrier limits of their fields. Acoustic metamaterial presents a unique solution as an artificially designed material showing low-frequency noise mitigation. A novel subwavelength device having thickness of 15mm (<2 cm) ,with potential application in noise mitigation and air ventilation solution is presented herein. In this study, the design and fabrication of a small prototype based on a Fresnel-spiral shape composed of several arms are performed. Numerical and experimental investigations were carried out to determine the acoustical properties of the proposed ventilated metamaterial in terms of sound absorption and sound transmission loss. The experimental investigation shows significant sound absorption with a high bandwidth (more than one octave in the range of > 900 Hz ), acoustic properties leading to potential applications in urban noise control for low and mid-frequency ranges.

Abstract The direct electrochemical nitric oxide reduction reaction (NORR) is an attractive technique for converting NO into NH 3 with low power consumption under ambient conditions. Optimizing the electronic structure of the active sites can greatly improve the performance of electrocatalysts. Herein, we prepare body‐centered cubic RuGa intermetallic compounds (i.e., bcc RuGa IMCs) via a substrate‐anchored thermal annealing method. The electrocatalyst exhibits a remarkable NH 4 + yield rate of 320.6 μmol h −1 mg −1 Ru with the corresponding Faradaic efficiency of 72.3 % at very low potential of −0.2 V vs. reversible hydrogen electrode (RHE) in neutral media. Theoretical calculations reveal that the electron‐rich Ru atoms in bcc RuGa IMCs facilitate the adsorption and activation of *HNO intermediate. Hence, the energy barrier of the potential‐determining step in NORR could be greatly reduced.

<p>Identification of BaC identity markers expressed in the basal compartment of healthy human breast. <b>A,</b> Schematic representation of a mammary gland duct cross-section, showing BaCs (blue) and LCs cells (red). ERα<sup>pos</sup> and ERα<sup>neg</sup> statuses are indicated by nuclear color. <b>B,</b> Integrated UMAP plot showing epithelial cell clustering from three murine scRNA-seq datasets. Color coding differentiates between BaCs (blue), ERα<sup>pos</sup> LC (red), and ERα<sup>neg</sup> LCs (green). <b>C,</b> UMAP plots showing the signature score of the top 20 DEGs associated with each epithelial compartment. Signature scores are color-coded from low (gray) to high (blue). <b>D,</b> UMAP plots showing expression levels of the top 10 BaC-associated genes across the integrated human dataset. Color gradient indicates expression levels from low (blue) to high (red). <b>E,</b> Representative IHC staining of breast tissue consecutive serial sections showing proteins expression of candidate BaC-associated genes. Scale bar, 50 μm. Arrows in the APOE panel highlight expression restricted to stromal cells. <b>A,</b> Created in BioRender. Rodilla, V. (2026) <a href="https://BioRender.com/qpb7bux" target="_blank">https://BioRender.com/qpb7bux</a>.</p>

Programmable acoustic metamaterials allow to manipulate sound waves at desirable frequency ranges intelligently, which makes it a viable and practical alternative to many noise cancellation applications. These kinds of metamaterials are amenable to intelligent programming and find place in a variety of real-life applications such as acoustic cloaking, noise cancellation, stealth applications, noise focusing, etc., simply with intelligent programming of the microstructure and the control of micro-movement of the various control boundaries instead of rebuilding new structures every time. Metamaterials are artificially designed materials that possess unique, unusual physical and mechanical properties, making them good lightweight candidates for various noise-shielding applications. However, many papers have been published on studying passive kinds of metamaterial structures whose material properties are fixed in space and time once designed and fabricated. This article is about a different class of metamaterials which are programmable and changeable through various control boundaries forming the structures for the applications pertaining to acoustic isolation (vibro-acoustics) and acoustic mitigation. The incorporation of shape memory polymers or smart polymers into the metamaterial structures enables fine and coarse tuning of these structures to the incoming noise frequency spectrum on a real-time basis. This article reviews some recent trends in progress made with smart materials and programmable metamaterials, their various programming techniques, the fundamental concept involved, various design strategies of such materials and their emergent applications in various fields of technology. The current fabrication challenges and future outlook in this promising field are also discussed.

The recent emergence of acoustic metamaterials presents unparalleled possibilities for sound control across diverse scenarios. However, achieving both sound absorption and unrestricted airflow concurrently in a one-dimensional scenario poses a challenge. Most available acoustic metamaterials are designed in 2D or 3D, and in these configurations, a large portion of noise is reflected back. The key challenge, therefore, is how to allow maximum air to pass through the structure, make it tunable and noise be effectively damped within it. To address this, we propose four different types of configurations as KF (Kink Fiber), YAM (“Y” Type kink Fiber), CDAM (Converging Diverging Kink Fiber), and IRAM (Internal Resonator Kink Fiber, a unique one-dimensional design that simultaneously overcomes both challenges. The design subwavelength (7 cm) one-dimensional acoustic meta-structured blanket, demonstrating broadband sound absorption bandwidth of one octave with 0.5-0.9 absorption within the 500-1600 Hz range. In this study, we theoretically (through transfer matrix method), numerically (through FEM), and experimentally (through Impedance tube method) have showcased that this challenge can be surmounted by employing various configurations of kink fiber-based metamaterials. Following the concept developed in this article there have been efforts to develop blankets using hand lay-up method which can be deployed in real-world setup based on one-dimensional acoustic damping concept introduced in this work.

The recent emergence of acoustic metamaterials presents unparalleled possibilities for sound control across diverse scenarios. However, achieving both sound absorption and unrestricted airflow concurrently in a one-dimensional scenario poses a challenge. Most available acoustic metamaterials are designed in 2D or 3D, and in these configurations, a large portion of noise is reflected back. The key challenge, therefore, is how to allow maximum air to pass through the structure, make it tunable and noise be effectively damped within it. To address this, we propose four different types of configurations as KF (Kink Fiber), YAM (“Y” Type kink Fiber), CDAM (Converging Diverging Kink Fiber), and IRAM (Internal Resonator Kink Fiber, a unique one-dimensional design that simultaneously overcomes both challenges. The design subwavelength (7 cm) one-dimensional acoustic meta-structured blanket, demonstrating broadband sound absorption bandwidth of one octave with 0.5–0.9 absorption within the 500–1600 Hz range. In this study, we theoretically (through transfer matrix method), numerically (through FEM), and experimentally (through Impedance tube method) have showcased that this challenge can be surmounted by employing various configurations of kink fiber-based metamaterials. Following the concept developed in this article there have been efforts to develop blankets using hand lay-up method which can be deployed in real-world setup based on one-dimensional acoustic damping concept introduced in this work.

<p>Analysis of basal marker expression and immune checkpoint genes in TNBC</p>

<p>Primary antibodies used in IF and WB assays</p>

<div>Abstract<p>Triple-negative breast cancer (TNBC) is the most heterogeneous and aggressive subtype of breast carcinoma, characterized by the absence of clinical biomarkers and the lack of targeted therapies. Despite numerous clinical trials, patient stratification remains suboptimal, limiting the identification of effective treatment strategies. In this study, we aimed to identify biomarkers exclusively expressed in the basal mammary epithelial compartment to refine TNBC subclassification. Computational analysis of single-cell RNA sequencing data defined a set of basal identity genes, which were subsequently validated by immunohistochemistry in two independent TNBC cohorts. This approach enabled the identification of a TNBC subgroup, termed true basal TNBC (tB-TNBC), which was associated with poorer prognosis. High-throughput screening of 3,200 FDA-approved compounds in breast cancer cell lines classified according to basal marker expression identified dasatinib as a promising candidate with selective activity against tB-TNBC models. In tB-TNBC patient-derived xenograft models, dasatinib treatment effectively suppressed tumor growth. Furthermore, <i>TAGLN</i> emerged as a strong predictive biomarker of dasatinib response, with functional studies confirming its role in modulating drug sensitivity. Altogether, these findings support the clinical utility of basal markers for TNBC stratification and highlight a targeted treatment opportunity for patients with tB-TNBC.</p>Significance:<p>Basal biomarkers enable subclassification of triple-negative breast cancer and reveal a specific tumor subgroup sensitive to dasatinib, providing an effective stratification and treatment strategy for patients.</p></div>

<p>Histologic expression of basal markers in breast tumor samples. <b>A,</b> Representative sections of tB- and nB-TNBC samples stained with the indicated BaC markers. Scale bar, 50 μm. <b>B,</b> Bar plots representing the percentage of cells expressing each marker in tB- and nB-TNBCs. *, <i>P</i> < 0.05 based on unpaired <i>t</i> test (Holm–Sidak method); ns, not significant. <b>C, </b>Left, representative images showing TAGL staining in tB- and nB-TNBC tumors. Scale bar, 200 and 50 μm in the insets.</p>

We present thin acoustic meta-structures with subwavelength dimensions through which almost perfect sound absorption is achieved in the low-frequency domain. Our overall strategy builds on the fact that the sound absorption capabilities of the meta-structures primarily depend on the geometric dimensions and can easily be reconfigured as per requirements through a change of geometry. To analyze various possibilities, we optimize the geometric structure through hybrid regression analysis using the genetic algorithm approach and finite element-based numerical simulations so that the geometry is tuned for high attenuation of acoustic signals over a broad range of frequencies. Both theoretical and experimental data show good parity and are able to establish the meta-structure nature of the assembly with respect to different frequency bands in the low frequency domain.

<p>Correlation between ACTA2, TAGLN, and TPM2 expression and drug sensitivity of selected compounds of drug screening</p>